bcl-x Antibody (BCL2L1/1762)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07416
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # BCL2L1/1762
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant human bcl-x protein fragment (around aa 1-135) (exact sequence is proprietary) (Uniprot: Q07817)
Localization
Cytoplasmic and cell / nuclear membrane
Specificity
Recognizes a protein of 27kDa, identified as the Bcl-X protein. This monoclonal antibody shows no cross-reaction with Bcl-2 or Bax protein. Bcl-X has two isoforms, Bcl-XL (long), a 241-amino acid protein which suppresses cell death. And Bcl-XS (short), a 178-amino acid protein lacking a 63-amino acid domain which functions as a dominant inhibitor of Bcl-2.
Marker
Apoptosis Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08580)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Applications for bcl-x Antibody (BCL2L1/1762)
Application
Recommended Usage
ELISA
1-5 ug/ml
Application Notes
ELISA: For coating use Ab at 1-5ug/ml, order Ab without BSA.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Bcl-x
Additional Bcl-x Products
Product Documents for bcl-x Antibody (BCL2L1/1762)
Product Specific Notices for bcl-x Antibody (BCL2L1/1762)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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