Key Product Details

Species Reactivity

Human, Mouse, Rat, Porcine

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # BX006

Format

IHC-Prediluted

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Bcl-x Antibodies »

Product Specifications

Immunogen

A synthetic peptide, aa 3-14 (Cys-QSNRELVVDFLS) of human Bcl-xL protein. (Uniprot: Q07817)

Localization

Cytoplasmic and cell/nuclear membrane

Specificity

Recognizes a protein of 27kDa, identified as the Bcl-X protein. This monoclonal antibody shows no cross-reaction with Bcl-2 or Bax protein. Bcl-X has two isoforms, Bcl-XL (long), a 241 amino acid protein which suppresses cell death. And Bcl-XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl-2. This monoclonal antibody reacts with both Bcl-XS and Bcl-XL proteins.

Marker

Apoptosis Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.

Scientific Data Images for bcl-x Antibody (BX006) - IHC-Prediluted

Immunohistochemistry-Paraffin: bcl-x Antibody (BX006) - IHC-Prediluted [NBP2-44413]

Immunohistochemistry-Paraffin: bcl-x Antibody (BX006) - IHC-Prediluted [NBP2-44413]

Immunohistochemistry-Paraffin: bcl-x Antibody (BX006) - IHC-Prediluted [NBP2-44413] - Formalin-paraffin Hodgkin's lymphoma stained with bcl-x antibody (BX006). Note cytoplasmic and membrane staining.

Applications for bcl-x Antibody (BX006) - IHC-Prediluted

Application
Recommended Usage

Immunohistochemistry-Paraffin

0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA buffer, pH 7.5-8.5, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Format

IHC-Prediluted

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Bcl-x

Bcl-x is a member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability. Alternative splicing results in two distinct Bcl-x isoforms. Bcl-xL (long) is an anti-apoptotic protein that prevents release of Cytochromec from the mitochondrial intermembrane space into the cytosol. Bcl-xs (short) is a pro-apoptotic member that can initiate programmed cell death.

Long Name

B Cell Lymphoma/Leukemia x

Alternate Names

BCL2L1, Bclx

Gene Symbol

BCL2L1

Additional Bcl-x Products

Product Documents for bcl-x Antibody (BX006) - IHC-Prediluted

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for bcl-x Antibody (BX006) - IHC-Prediluted

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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