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bcl-x Antibody (rBCL2L1/4508) [Janelia Fluor® 669]

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14164JF669

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry-Paraffin

Label

Janelia Fluor 669

Antibody Source

Recombinant Monoclonal Mouse IgG2a Kappa Clone # rBCL2L1/4508

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for bcl-x Antibody (rBCL2L1/4508) [Janelia Fluor® 669]

Immunogen

Recombinant fragment (around aa1-100) of human bcl-x protein (exact sequence is proprietary) (Uniprot: Q07817 )

Localization

Cytoplasm, Cell membrane, Nuclear membrane

Specificity

Recognizes a protein of 27kDa, identified as the Bcl-X protein. This monoclonal antibody shows no cross-reaction with Bcl-2 or Bax protein. Bcl-X has two isoforms, Bcl-XL (long), a 241-amino acid protein which suppresses cell death. And Bcl-XS (short), a 178-amino acid protein lacking a 63-amino acid domain which functions as a dominant inhibitor of Bcl-2.

Marker

Apoptosis Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Applications for bcl-x Antibody (rBCL2L1/4508) [Janelia Fluor® 669]

Application
Recommended Usage

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Bcl-x

Bcl-x is a member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability. Alternative splicing results in two distinct Bcl-x isoforms. Bcl-xL (long) is an anti-apoptotic protein that prevents release of Cytochromec from the mitochondrial intermembrane space into the cytosol. Bcl-xs (short) is a pro-apoptotic member that can initiate programmed cell death.

Long Name

B Cell Lymphoma/Leukemia x

Alternate Names

BCL2L1, Bclx

Gene Symbol

BCL2L1

Additional Bcl-x Products

Product Documents for bcl-x Antibody (rBCL2L1/4508) [Janelia Fluor® 669]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for bcl-x Antibody (rBCL2L1/4508) [Janelia Fluor® 669]



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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