Bcl-xL Antibody (016)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89507

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 016

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Bcl-xL Antibodies »

Product Summary for Bcl-xL Antibody (016)

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human Bcl-xL (Accession#: NP_612815.1; Met1-Arg212).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Bcl-xL Antibody (016)

Western Blot: Bcl-xL Antibody (016) [NBP2-89507]

Western Blot: Bcl-xL Antibody (016) [NBP2-89507]

Western Blot: Bcl-xL Antibody (016) [NBP2-89507] - Lane A: HepG2 Whole Cell Lysate Lane B: K562 Whole Cell LysateLane C: NIH-3T3 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:26 kDa Observed band size:30 kDa
Immunoprecipitation: Bcl-xL Antibody (016) [NBP2-89507]

Immunoprecipitation: Bcl-xL Antibody (016) [NBP2-89507]

Immunoprecipitation: Bcl-xL Antibody (016) [NBP2-89507] - Lane A:0.5 mg Jurkat Whole Cell Lysate Lane B:0.5 mg K562 Whole Cell Lysate1 uL anti-BCL2L1 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-BCL2L1 rabbit monoclonal antibody,at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 26 kDa Observed band size: 26 kDa

Applications for Bcl-xL Antibody (016)

Application
Recommended Usage

ELISA

1:25000-1:50000

Immunoprecipitation

0.2-1 uL/mg of lysate

Western Blot

1:500-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Bcl-xL

Bcl-x, a member of the Bcl-2 protein family, inhibits cell death, or apoptosis (1). Bcl-x is expressed as two isomeric forms, Bcl-xL and Bcl-xS, and is typically present in the cytosol in association with the mitochondrial membrane. Bcl-xL forms heterodimers with various proteins, including Bax, Bak and Bcl-2 (2). It has been found that eterodimerization with Bax does not seem to be required for anti-apoptotic activity (3). Since Bcl-xL can form an ion channel in synthetic lipid membranes, there is a strong possibility that this property plays a role in heterodimerization-independent cell survival (4). The Bcl-X(S) isoform promotes apoptosis.

Long Name

B Cell Lymphoma/Leukemia x Long Form

Alternate Names

BclxL

Gene Symbol

BCL2L1

Additional Bcl-xL Products

Product Documents for Bcl-xL Antibody (016)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Bcl-xL Antibody (016)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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