Bcl-xL Antibody (7B2.5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-28559

Novus Biologicals, part of Bio-Techne
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NBP1-28559
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Biotin
NBP1-28561
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Rhesus Macaque

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunoprecipitation, Western Blot

Cited:

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Kappa Clone # 7B2.5

Concentration

0.1 mg/ml

View all Bcl-xL Antibodies »

Product Specifications

Immunogen

Recombinant human Bcl-xS

Specificity

Human/Mouse/Rat/Rhesus Bcl-xL

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3 Kappa

Scientific Data Images for Bcl-xL Antibody (7B2.5)

Flow Cytometry: Bcl-xL Antibody (7B2.5) [NBP1-28559]

Flow Cytometry: Bcl-xL Antibody (7B2.5) [NBP1-28559]

Flow Cytometry: Bcl-xL Antibody (7B2.5) [NBP1-28559] - Human T cell leukemia cell line Jurkat was intracellularly stained with Mouse Anti-Human Bcl-xL-FITC (NBP1-28560).
Flow Cytometry: Bcl-xL Antibody (7B2.5) [NBP1-28559]

Flow Cytometry: Bcl-xL Antibody (7B2.5) [NBP1-28559]

Flow Cytometry: Bcl-Xl Antibody (7B2.5) [NBP1-28559] - Bcl-Xl Antibody (7B2.5) 2 ug/10^6 Murine FL5 cells (FL5-NEO) and FL5 cells transfected with Bcl-xL expression plasmid (FL5-BCL-XL) were fixed with buffered paraformaldehyde and then permeabilized with saponin. The cells were incubated with mouse anti-human Bcl-xL followed by phycoerythrin-conjugated goat anti-mouse IgG, and then were analyzed by flow cytometry.
Flow Cytometry: Bcl-xL Antibody (7B2.5) [NBP1-28559]

Flow Cytometry: Bcl-xL Antibody (7B2.5) [NBP1-28559]

Flow Cytometry: Bcl-xL Antibody (7B2.5) [NBP1-28559] - T Cell leukemia dell line Jurkat was intracellularly stained with Mouse Anti-Human.

Applications for Bcl-xL Antibody (7B2.5)

Application
Recommended Usage

Flow Cytometry

< = 3 ug/10^6 cells

Immunoprecipitation

1:10-1:500
Application Notes
Use in WB reported in scientific literature (PMID:33353284). IP reactivity reported in (PMID: 26939706).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

BBS (pH 8.2)

Preservative

No Preservative

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Bcl-xL

Apoptosis, or programmed cell death, is a well-documented phenomenon in many cellular systems. (1) It plays a key role in tissue and organ development as well as in adult tissues during cell turnover. Apoptosis can be induced by a variety of internal and external stimuli including growth factor deprivation, cytokine treatment, antigen-receptor engagement, cell-cell interactions, irradiation and glucocorticoid treatment. (2) Bcl-2 and one of its homologues, Bcl-xL, protect cells from apoptosis (3,4), while other homologues of Bcl-2 such as Bax, Bad and Bak have been shown to enhance apoptosis. (5-8) Bcl-xL has been shown to block apoptosis which is induced by a variety of stimuli and, under certain conditions, offers greater protection against apoptosis than Bcl-2. (9-13) In contrast, Bad and Bax inhibit the protective functions of Bcl-xL and Bcl-2, respectively. Although heterodimerization between Bcl-xL/Bad and Bcl-2/Bax was originally thought to be essential for the differential anti-apoptotic activity of Bcl-xL and Bcl-2, (5,14) other results suggest that the formation of heterodimers may not be necessary for this death-repressing activity. (15,16) Mab 7B2.5 recognizes both human and rodent Bcl-xL. (17)

Long Name

B Cell Lymphoma/Leukemia x Long Form

Alternate Names

BclxL

Gene Symbol

BCL2L1

Additional Bcl-xL Products

Product Documents for Bcl-xL Antibody (7B2.5)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Bcl-xL Antibody (7B2.5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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