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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human Bcl-B. The immunogen is located within amino acids 90 - 140 of Bcl-B. Amino Acid Squence: RWKKWGFQPRLKEQEGD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BCL2L10 Antibody - BSA Free

Western Blot: BCL2L10 AntibodyBSA Free [NBP1-77224]

Western Blot: BCL2L10 AntibodyBSA Free [NBP1-77224]

Western Blot: BCL2L10 Antibody [NBP1-77224] - Analysis of Bcl-B in Jurkat cell lysate with Bcl-B antibody at (A) 1 and (B) 2 ug/mL.
Immunocytochemistry/ Immunofluorescence: BCL2L10 Antibody - BSA Free [NBP1-77224]

Immunocytochemistry/ Immunofluorescence: BCL2L10 Antibody - BSA Free [NBP1-77224]

Immunocytochemistry/Immunofluorescence: BCL2L10 Antibody [NBP1-77224] - Jurkat cells with BCL2L10 antibody at 10 ug/ml.
BCL2L10 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: BCL2L10 Antibody - BSA Free [NBP1-77224] -

Immunocytochemistry/ Immunofluorescence: BCL2L10 Antibody - BSA Free [NBP1-77224] - Immunocytochemistry of BCL2L10 in Jurkat cells with BCL2L10 antibody at 10 u/mL.

Applications for BCL2L10 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Despite its predicted molecular weight, BCL2L10 is often at higher molecular weights, presumably due to post-translational modifications.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BCL2L10

Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-B is a recently discovered anti-apoptotic member of the Bcl-2. Unlike the mouse homolog (also known as Diva/Boo) which is predominantly expressed in ovary and testis, the human Bcl-B protein is widely expressed. Also, the human Bcl-B functions by binding to and suppressing the apoptotic activity of Bax, whereas the mouse homolog binds Bak and also interacts with the apoptosis protein Apaf-1. Despite its predicted molecular weight, Bcl-B is often at higher molecular weights, presumably due to post-translational modifications.

Long Name

Bcl-2-like protein 10

Alternate Names

BCL-B, Bcl2-L-10, BCLB, BOO, DIVA

Gene Symbol

BCL2L10

Additional BCL2L10 Products

Product Documents for BCL2L10 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for BCL2L10 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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