BCR Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-19097

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

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Product Summary for BCR Antibody

Immunogen

The immunogen recognized by this antibody maps to a region between residue 50 and 100 of human breakpoint cluster region using the numbering given in entry NP_004318.3 (GeneID 613).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NBP1-19098, NB200-171

Scientific Data Images for BCR Antibody

Immunocytochemistry/ Immunofluorescence: BCR Antibody [NBP1-19097]

Immunocytochemistry/ Immunofluorescence: BCR Antibody [NBP1-19097]

Immunocytochemistry/Immunofluorescence: BCR Antibody [NBP1-19097] - Human K562 cells (contains the chromosomal translocation, t(9:22) that creates the BCR/ABL fusion gene). Antibody used at a dilution of 1:1,000 (1ug/ml). Detection: Green-fluorescent goat anti-rabbit IgG - AlexaFluor 488 used at a dilution of 1:1,000.
Flow Cytometry: BCR Antibody [NBP1-19097]

Flow Cytometry: BCR Antibody [NBP1-19097]

Flow Cytometry: BCR Antibody [NBP1-19097] - Flow Cytometric Detection of BCR. 1 x 106 K562 cells were fixed, permeabilized, and stained with 0.25 mcg anti-BCR in a 150 mcl reaction. Isotype anti-KLH control (black), no antibody (red) and anti-BCR (blue).
Immunoprecipitation: BCR Antibody [NBP1-19097]

Immunoprecipitation: BCR Antibody [NBP1-19097]

Immunoprecipitation: BCR Antibody [NBP1-19097] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-BCR antibody used for IP at 10 mcg/mg lysate. BCR was also immunoprecipitated by rabbit anti-BCR antibody NBP1-19098, which recognizes a downstream epitope

Applications for BCR Antibody

Application
Recommended Usage

Flow Cytometry

0.25 mcg per 1X10e6 cells in 150 mcl volume

Immunocytochemistry/ Immunofluorescence

1:500 - 1:2000

Immunoprecipitation

10 ug/mg lysate
Application Notes
Western Blot Not recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: BCR

A reciprocal translocation between chromosomes 22 and 9 produces the Philadelphia chromosome, which is often found in patients with chronic myelogenous leukemia. The chromosome 22 breakpoint for this translocation is located within the BCR gene. The translocation produces a fusion protein which is encoded by sequence from both BCR and ABL, the gene at the chromosome 9 breakpoint. Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for p21rac. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Long Name

Breakpoint Cluster Region

Alternate Names

ALL, PHL

Entrez Gene IDs

613 (Human)

Gene Symbol

BCR

UniProt

NP_004318.3 (Human)

Additional BCR Products

Product Documents for BCR Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for BCR Antibody

This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NBP1-19098, NB200-171

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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