BDH1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88673

Novus Biologicals, part of Bio-Techne
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NBP1-88673
NBP1-88673-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Rat

Cited:

Mouse, Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all BDH1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ATSLYSPESIQAIAKKMWEELPEVVRKDYGKKYFDEKIAKMETYCSSGSTDTSPVIDAVTHALTATTPYTRYHPMDYYWWLRMQIMTHL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BDH1 Antibody

Immunohistochemistry-Paraffin: BDH1 Antibody [NBP1-88673]

Immunohistochemistry-Paraffin: BDH1 Antibody [NBP1-88673]

Immunohistochemistry-Paraffin: BDH1 Antibody [NBP1-88673] - Analysis in human liver and skeletal muscle tissues using antibody. Corresponding BDH1 RNA-seq data are presented for the same tissues.
Western Blot: BDH1 Antibody [NBP1-88673]

Western Blot: BDH1 Antibody [NBP1-88673]

Western Blot: BDH1 Antibody [NBP1-88673] - Analysis in human liver tissue.
Immunocytochemistry/ Immunofluorescence: BDH1 Antibody [NBP1-88673]

Immunocytochemistry/ Immunofluorescence: BDH1 Antibody [NBP1-88673]

Immunocytochemistry/Immunofluorescence: BDH1 Antibody [NBP1-88673] - Staining of human cell line U-251 MG shows localization to nucleoplasm and mitochondria. Antibody staining is shown in green.
View 5 more images

Applications for BDH1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Frozen

Reported in scientific literature (PMID:35902007).

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BDH1

BDH1 encodes a member of the short-chain dehydrogenase/reductase gene family. The encoded protein forms a homotetrameric lipid-requiring enzyme of the mitochondrial membrane and has a specific requirement for phosphatidylcholine for optimal enzymatic activity. The encoded protein catalyzes the interconversion of acetoacetate and (R)-3-hydroxybutyrate, the two major ketone bodies produced during fatty acid catabolism. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq]

Alternate Names

(R)-3-hydroxybutyrate dehydrogenase, 3-hydroxybutyrate dehydrogenase, 3-hydroxybutyrate dehydrogenase, type 1, EC 1.1.1, MGC2723, MGC4347, MGC9788, mitochondrial, mitochondrial), short chain dehydrogenase/reductase family 9C, member 1

Gene Symbol

BDH1

Additional BDH1 Products

Product Documents for BDH1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for BDH1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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