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BDNF Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-59304

Novus Biologicals, part of Bio-Techne
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NBP1-59304

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Monkey, Rhesus Macaque

Cited:

Mouse, Primate - Macaca mulatta (Rhesus Macaque)

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Synthetic peptides corresponding to BDNF(brain-derived neurotrophic factor) The peptide sequence was selected from the middle region of BDNF. Peptide sequence EWVTAADKKTAVDMSGGTVTVLEKVPVSKGQLKQYFYETKCNPMGYTKEG. The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for BDNF Antibody

Western Blot: BDNF Antibody [NBP1-59304]

Western Blot: BDNF Antibody [NBP1-59304]

Western Blot: BDNF Antibody [NBP1-59304] - ANT-DBS inhibited BDNF-TrkB pathway and its downstream regulator in the hippocampus of epileptic monkeys. Analysis of BDNF (1:1000, NBP1-59304), TrkB (1:500, NPB1-47898), p-ERK, ERK, p-Akt, Akt, p-S6K and S6K by western blotting. Image collected and cropped by CiteAb from the following publication (www.aging-us.com/lookup/doi/10.18632/aging.103026) licensed under a CC-BY license.
Immunohistochemistry: BDNF Antibody [NBP1-59304]

Immunohistochemistry: BDNF Antibody [NBP1-59304]

Immunohistochemistry: BDNF Antibody [NBP1-59304] - Ventral horn region of mouse spinal cord. Concentration 1:200
Western Blot: BDNF Antibody [NBP1-59304]

Western Blot: BDNF Antibody [NBP1-59304]

Western Blot: BDNF Antibody [NBP1-59304] - ACHN Whole Cell lysates.

Applications for BDNF Antibody

Application
Recommended Usage

Immunohistochemistry

1:200- 1:500

Western Blot

1.0 ug/ml
Application Notes
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID:31849603).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BDNF

BDNF is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression BDNF is reduced in both Alzheimer's and Huntington disease patients. BDNF may play a role in the regulation of stress response and in the biology of mood disorders.The protein encoded by this gene is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer's and Huntington disease patients. This gene may play a role in the regulation of stress response and in the biology of mood disorders. Multiple transcript variants encoding distinct isoforms have been described for this gene, but the full-length nature of only some could be determined.

Long Name

Brain-derived Neurotrophic Factor

Alternate Names

Abrineurin, ANON2, BULN2, Neurotrophin

Gene Symbol

BDNF

UniProt

Additional BDNF Products

Product Documents for BDNF Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for BDNF Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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