BDNF Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-59304
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Monkey, Rhesus Macaque
Cited:
Mouse, Primate - Macaca mulatta (Rhesus Macaque)
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Synthetic peptides corresponding to BDNF(brain-derived neurotrophic factor) The peptide sequence was selected from the middle region of BDNF. Peptide sequence EWVTAADKKTAVDMSGGTVTVLEKVPVSKGQLKQYFYETKCNPMGYTKEG. The peptide sequence for this immunogen was taken from within the described region.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
Scientific Data Images for BDNF Antibody
Western Blot: BDNF Antibody [NBP1-59304]
Western Blot: BDNF Antibody [NBP1-59304] - ANT-DBS inhibited BDNF-TrkB pathway and its downstream regulator in the hippocampus of epileptic monkeys. Analysis of BDNF (1:1000, NBP1-59304), TrkB (1:500, NPB1-47898), p-ERK, ERK, p-Akt, Akt, p-S6K and S6K by western blotting. Image collected and cropped by CiteAb from the following publication (www.aging-us.com/lookup/doi/10.18632/aging.103026) licensed under a CC-BY license.Immunohistochemistry: BDNF Antibody [NBP1-59304]
Immunohistochemistry: BDNF Antibody [NBP1-59304] - Ventral horn region of mouse spinal cord. Concentration 1:200Western Blot: BDNF Antibody [NBP1-59304]
Western Blot: BDNF Antibody [NBP1-59304] - ACHN Whole Cell lysates.Applications for BDNF Antibody
Application
Recommended Usage
Immunohistochemistry
1:200- 1:500
Western Blot
1.0 ug/ml
Application Notes
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID:31849603).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 2% Sucrose
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: BDNF
Long Name
Brain-derived Neurotrophic Factor
Alternate Names
Abrineurin, ANON2, BULN2, Neurotrophin
Gene Symbol
BDNF
UniProt
Additional BDNF Products
Product Documents for BDNF Antibody
Product Specific Notices for BDNF Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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