BDNF Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-00529
Key Product Details
Species Reactivity
Human
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
This IgG fraction BDNF Antibody was prepared from rabbit antiserum after repeated immunizations with recombinant truncated human BDNF protein produced in E.coli. (Uniprot: P23560)
Reactivity Notes
Based on high to 100% homology, there is a chance of cross-reactivity to BDNF from a wide variety of animals. Cross-reactivity with BDNF from other sources has not been determined
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for BDNF Antibody
Western Blot: BDNF Antibody [NBP3-00529]
Western Blot: BDNF Antibody - Detection of recombinant human BDNF raised in E.coli.Applications for BDNF Antibody
Application
Recommended Usage
ELISA
1:10000
Western Blot
1:1000
Application Notes
This purified antibody has been tested in western blotting and suitable for ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 27 kDa in size corresponding to the mature human BDNF protein by western blotting in appropriate cell lysate or extract.
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 100 ul deionized water (or equivalent)
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: BDNF
Long Name
Brain-derived Neurotrophic Factor
Alternate Names
Abrineurin, ANON2, BULN2, Neurotrophin
Gene Symbol
BDNF
UniProt
Additional BDNF Products
Product Documents for BDNF Antibody
Product Specific Notices for BDNF Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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