Beclin 1 Antibody (JE59-31)

Immunohistochemistry: Beclin 1 Antibody (JE59-31) [NBP3-32076]

Immunohistochemistry: Beclin 1 Antibody (JE59-31) [NBP3-32076] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Beclin 1 antibody (NBP3-32076) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32076) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence: Beclin 1 Antibody (JE59-31) [NBP3-32076]

Immunocytochemistry/ Immunofluorescence: Beclin 1 Antibody (JE59-31) [NBP3-32076] - Immunocytochemistry analysis of Raji cells labeling Beclin 1 with Rabbit anti-Beclin 1 antibody (NBP3-32076) at 1/250 dilution.

Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Beclin 1 antibody (NBP3-32076) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.