Beclin 1 [p Ser15] Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16954
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic phosphorylated peptide around S15 of human Beclin 1 (NP_003757.1). QVSFV
Modification
p Ser15
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Beclin 1 [p Ser15] Antibody - Azide and BSA Free
![Western Blot: Beclin 1 [p Ser15] AntibodyAzide and BSA Free [NBP3-16954]](https://resources.bio-techne.com/images/products/Beclin-1-[p-Ser15]-Antibody-Western-Blot-NBP3-16954-img0001.jpg "Western Blot: Beclin 1 [p Ser15] AntibodyAzide and BSA Free [NBP3-16954]")
Western Blot: Beclin 1 [p Ser15] AntibodyAzide and BSA Free [NBP3-16954]
Western Blot: Beclin 1 [p Ser15] Antibody [NBP3-16954] - Analysis of extracts of various cell lines, using Beclin 1 antibody (NBP3-16954) at 1:500 dilution.NIH/3T3 and C6 cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.Applications for Beclin 1 [p Ser15] Antibody - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:100 - 1:500
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 50% glycerol, pH7.3
Format
Azide and BSA Free
Preservative
0.05% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Beclin 1
Beclin-1 encodes an evolutionarily conserved 60kDa coiled-coil protein that is expressed in human muscle, epithelial cells and neurons. In gene transfer studies, beclin 1 promotes nutrient deprivation-induced autophagy, inhibits mammary tumorigenesis, and inhibits viral replication.
Expression of the Beclin1 protein is frequently decreased in malignant breast epithelial cells. Based upon these observations, it is speculated that beclin-1 may work through induction of autophagy to negatively regulate tumorigenesis and to control viral infections. Beclin 1 may also play a role in other biological processes in which autophagy is important such as cell differentiation and nutritional stress responses.
Long Name
Beclin1, Autophagy-related
Alternate Names
ATG6, BECN1, GT197, VPS30
Gene Symbol
BECN1
Additional Beclin 1 Products
Product Documents for Beclin 1 [p Ser15] Antibody - Azide and BSA Free
Product Specific Notices for Beclin 1 [p Ser15] Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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