Key Product Details

Species Reactivity

Validated:

Human, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

View all Beclin 2 Antibodies »

Product Summary for Beclin 2 Antibody

Immunogen

Synthetic peptide from the C-terminal of Human Beclin 2 (aa. 338-349)

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Beclin 2 Antibody

Western Blot: Beclin 2 Antibody [NBP3-18252]

Western Blot: Beclin 2 Antibody [NBP3-18252]

Western Blot: Beclin 2 Antibody [NBP3-18252] - Western blot analysis of Rat Liver cell lysates showing detection of 48.1 kDa Beclin 2 protein using Rabbit Anti-Beclin 2 Polyclonal Antibody (NBP3-18252). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Liver cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Beclin 2 Polyclonal Antibody (NBP3-18252) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 48.1 kDa.
Immunocytochemistry/ Immunofluorescence: Beclin 2 Antibody [NBP3-18252]

Immunocytochemistry/ Immunofluorescence: Beclin 2 Antibody [NBP3-18252]

Immunocytochemistry/Immunofluorescence: Beclin 2 Antibody [NBP3-18252] - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Beclin 2 Polyclonal Antibody (NBP3-18252). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Beclin 2 Polyclonal Antibody (NBP3-18252) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Beclin 2 Antibody (D) Composite.

Applications for Beclin 2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000
Application Notes
Optimal dilutions for assays should be determined by the user.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS, 50% glycerol

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Beclin 2

Beclin 2, also known as Beclin-1-like-1 is a novel coiled-coil protein related to the autophagic Beclin 1 protein. Beclin 2 is thought to interact with Bcl-2, an anti-apoptotic protein, and is believed to function in autophagy and antiviral host defense.

Alternate Names

BECN1 L1, BECN1L1, BECN1P1, BECN1P1 beclin 1, autophagy related, pseudogene 1

Gene Symbol

BECN2

Additional Beclin 2 Products

Product Documents for Beclin 2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Beclin 2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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