beta 2-Microglobulin Antibody (246-E9.E7 (same as HLA.ABC.m2))
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44465
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Primate (Negative)
Applications
Flow Cytometry, Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 246-E9.E7 (same as HLA.ABC.m2)
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Human PBL s from a T-cell acute lymphoblastic leukemia (T-ALL) patient
Reactivity Notes
Does not react with Non-Human primates.
Localization
Cell Surface and Extracellular (Secreted)
Specificity
Recognizes a protein of 12kDa, identified as beta-microglobulin. Beta--microglobulin non-covalently associates with the 44kDa chain to form the HLA Class I antigen complex. Human beta-2 microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes. This monoclonal antibody is specific to human beta-2 microglobulin and does not react with non-human primate cells. This antibody reacts with all cell types excluding erythrocytes. Detection of beta-2 microglobulin in body fluids has been used as a tumor marker and for monitoring patients with HIV infection.
Marker
Renal Failure & Tumor Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Theoretical MW
12 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47700)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Applications for beta 2-Microglobulin Antibody (246-E9.E7 (same as HLA.ABC.m2))
Application
Recommended Usage
Flow Cytometry
0.5 - 1 ug/million cells in 0.1 ml
Immunofluorescence
0.5 - 1.0 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: beta 2-Microglobulin
Additional beta 2-Microglobulin Products
Product Documents for beta 2-Microglobulin Antibody (246-E9.E7 (same as HLA.ABC.m2))
Product Specific Notices for beta 2-Microglobulin Antibody (246-E9.E7 (same as HLA.ABC.m2))
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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