beta 2-Microglobulin Antibody (3F9-2C2)

Novus Biologicals, part of Bio-Techne | Catalog # H00000567-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Proximity Ligation Assay, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 3F9-2C2

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all beta 2-Microglobulin Antibodies »

Product Specifications

Immunogen

B2M (AAH32589, 1 a.a. ~ 119 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSRSVALAVLALLSLSGLEAIQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM

Specificity

B2M - beta-2-microglobulin

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for beta 2-Microglobulin Antibody (3F9-2C2)

Western Blot: beta 2-Microglobulin Antibody (3F9-2C2) [H00000567-M01]

Western Blot: beta 2-Microglobulin Antibody (3F9-2C2) [H00000567-M01]

Western Blot: beta 2-Microglobulin Antibody (3F9-2C2) [H00000567-M01] - Analysis of B2M expression in transfected 293T cell line by B2M monoclonal antibody (M01), clone 3F9-2C2.Lane 1: B2M transfected lysate(13.7 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: beta 2-Microglobulin Antibody (3F9-2C2) [H00000567-M01]

Immunocytochemistry/ Immunofluorescence: beta 2-Microglobulin Antibody (3F9-2C2) [H00000567-M01]

Immunocytochemistry/Immunofluorescence: beta 2-Microglobulin Antibody (3F9-2C2) [H00000567-M01] - Analysis of monoclonal antibody to B2M on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (3F9-2C2) [H00000567-M01]

Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (3F9-2C2) [H00000567-M01]

Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (3F9-2C2) [H00000567-M01] - Analysis of monoclonal antibody to B2M on formalin-fixed paraffin-embedded human esophagus. Antibody concentration 3 ug/ml.
View 4 more images

Applications for beta 2-Microglobulin Antibody (3F9-2C2)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactivity against recominant protein and cell lysate for WB. It has been used for IF, IHC-P, RNAi Validation and ELISA.Use in Immunohistochemistry reported in scientific literature (PMID: 24736453).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: beta 2-Microglobulin

Beta-2-microglobulin is a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells (Gussow et al., 1987 [PubMed 3312414]).[supplied by OMIM]

Alternate Names

B2M, beta 2Microglobulin

Entrez Gene IDs

567 (Human)

Gene Symbol

B2M

OMIM

109700 (Human)

UniProt

AAH32589 (Human)

Additional beta 2-Microglobulin Products

Product Documents for beta 2-Microglobulin Antibody (3F9-2C2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for beta 2-Microglobulin Antibody (3F9-2C2)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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