beta 2-Microglobulin Antibody (4G5A1) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37284

Novus Biologicals, part of Bio-Techne
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NBP2-37284
NBP2-37284-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human

Applications

CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4G5A1

Format

BSA Free

Concentration

1 mg/ml

View all beta 2-Microglobulin Antibodies »

Product Summary for beta 2-Microglobulin Antibody (4G5A1) - BSA Free

Immunogen

Purified recombinant fragment of human beta 2-Microglobulin (AA: 21-100) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

13.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for beta 2-Microglobulin Antibody (4G5A1) - BSA Free

Western Blot: beta 2-Microglobulin Antibody (4G5A1)BSA Free [NBP2-37284]

Western Blot: beta 2-Microglobulin Antibody (4G5A1)BSA Free [NBP2-37284]

Western Blot: beta 2-Microglobulin Antibody (4G5A1) [NBP2-37284] - Western blot analysis using B2M mAb against HEK293 (1) and B2M (AA: 21-100)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: beta 2-Microglobulin Antibody (4G5A1) - BSA Free [NBP2-37284]

Immunocytochemistry/ Immunofluorescence: beta 2-Microglobulin Antibody (4G5A1) - BSA Free [NBP2-37284]

Immunocytochemistry/Immunofluorescence: beta 2-Microglobulin Antibody (4G5A1) [NBP2-37284] - Immunofluorescence analysis of Hela cells using B2M mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.
Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (4G5A1) - BSA Free [NBP2-37284]

Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (4G5A1) - BSA Free [NBP2-37284]

Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (4G5A1) [NBP2-37284] - Analysis of paraffin-embedded cervical cancer tissues using B2M mouse mAb with DAB staining.
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Applications for beta 2-Microglobulin Antibody (4G5A1) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: beta 2-Microglobulin

Beta-2-microglobulin is a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells (Gussow et al., 1987 [PubMed 3312414]).[supplied by OMIM]

Alternate Names

B2M, beta 2Microglobulin

Gene Symbol

B2M

Additional beta 2-Microglobulin Products

Product Documents for beta 2-Microglobulin Antibody (4G5A1) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for beta 2-Microglobulin Antibody (4G5A1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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