beta-Actin Antibody (CL3868)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-76481

Novus Biologicals, part of Bio-Techne
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NBP2-76481
NBP2-76481-25ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL3868

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all beta-Actin Antibodies »

Product Specifications

Immunogen

This beta-Actin Antibody (CL3868) was developed against a recombinant protein corresponding to amino acids: PKANREKMTQIMFETFNTPAMYVAIQ

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for beta-Actin Antibody (CL3868)

Western Blot: beta-Actin Antibody (CL3868) [NBP2-76481]

Western Blot: beta-Actin Antibody (CL3868) [NBP2-76481]

Western Blot: beta-Actin Antibody (CL3868) [NBP2-76481] - Lane 1: Molecular weight marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human cell line HeLa. Lane 3: Human cell line HEK 293. Lane 4: Human cell line A-431. Lane 5: Human cell line HepG2. Lane 6: Mouse cell line NIH-3T3. Lane 7: Rat cell line NBT-II.
Immunohistochemistry-Paraffin: beta-Actin Antibody (CL3868) [NBP2-76481]

Immunohistochemistry-Paraffin: beta-Actin Antibody (CL3868) [NBP2-76481]

Immunohistochemistry-Paraffin: beta-Actin Antibody (CL3868) [NBP2-76481] - Staining of human tonsil shows strong positivity in lymphoid cells.
Immunohistochemistry-Paraffin: beta-Actin Antibody (CL3868) [NBP2-76481]

Immunohistochemistry-Paraffin: beta-Actin Antibody (CL3868) [NBP2-76481]

Immunohistochemistry-Paraffin: beta-Actin Antibody (CL3868) [NBP2-76481] - Staining of human prostate shows strong cytoplasmic immunoreactivity in smooth muscle and weak positivity in glandular cells.
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Applications for beta-Actin Antibody (CL3868)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:5000 - 1:10000

Immunohistochemistry-Paraffin

1:5000 - 1:10000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, pH 7.2, containing 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: beta-Actin

Actin is a highly conserved protein involved in cell motility, structure, and integrity. Six functional actin isoforms exist and were named based on their electrophoretic mobility and distribution including alpha-cardiac muscle actin (aCAA), alpha-skeletal muscle actin (aSKA), alpha-smooth muscle actin (aSMA), beta-cytoplasmic actin (bCYA), gamma-cytoplasmic actin (gCYA) and gamma-smooth muscle actin (gSMA) (1). The cytoplasmic isoforms, beta-actin and gamma-actin, are nearly identical, differing by only 4 amino acids at the N-terminus with a theoretical molecular weight of 42 kDa (2). Beta-actin is ubiquitously expressed in eukaryotic cells and considered a housekeeping gene which is frequently used as a loading control for assays involving protein detection, such as Western blotting (3,4).

References

1. Vandekerckhove J, Weber K. 1978. At least six different actins are expressed in a higher mammal: an analysis based on the amino acid sequence of the amino-terminal tryptic peptide. J Mol Biol. 126(4):783-802. PMID: 745245

2. Gimona M, Vandekerckhove J, Goethals M, Herzog M, Lando Z, Small JV. (1994) Beta-actin specific monoclonal antibody. Cell Motil Cytoskeleton. 27(2):108-16. PMID: 8162619

3. Holden VI, Lenio S, Kuick R, Ramakrishnan SK, Shah YM, Bachman MA. (2014) Bacterial siderophores that evade or overwhelm Lipocalin 2 induce HIF-1a and pro-inflammatory cytokine secretion in cultured respiratory epithelial cells Infect Immun. 82(9):3826-36 PMID: 24980968

4. Tsai WL, Yeh PH, Tsai CY, Ting CT, Chiu YH, Tao MH, Li WC, Hung SC. (2016) Efficient Programming of Human Mesenchymal Stem Cell Derived Hepatocytes by Epigenetic Regulations. J. Gastroenterol. Hepatol. 32(1):261-269. PMID: 27218433

Alternate Names

ACTB, betaActin

Gene Symbol

ACTB

Additional beta-Actin Products

Product Documents for beta-Actin Antibody (CL3868)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for beta-Actin Antibody (CL3868)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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