beta-Actin Antibody (M008)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23324

Novus Biologicals, part of Bio-Techne
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NBP3-23324
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # M008

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all beta-Actin Antibodies »

Product Specifications

Immunogen

Clone (M008) was generated from a proprietary antigen related to human beta-Actin in MDA-MB-231 breast cancer cell line. [UniProt# P60709]

Specificity

Clone M008 detects a 42 kDa* protein corresponding to the molecular mass of beta-actin on SDS-PAGE immunoblots of human cancer cell lines, as well as human recombinant beta actin. This actin antibody preferentially detects human beta-actin with only weak reactivity toward actins in rat, mouse, or rabbit.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for beta-Actin Antibody (M008)

beta-Actin Antibody (M008)

Western Blot: beta-Actin Antibody (M008) [NBP3-23324] -

Western Blot: beta-Actin Antibody (M008) [NBP3-23324] - Western blot analysis of human HUVEC-CS (lane 1), rabbit spleen fibroblast (lane 2), human Jurkat (lane 3), human LNCaP (lane 4), human HeLa (lane 5), and mouse F9 (lane 6) cell lysates. The blot was probed with mouse monoclonal anti-beta-Actin (NBP3-23324) at 1:1000 (lanes 1-6).
beta-Actin Antibody (M008)

Immunocytochemistry/Immunofluorescence: beta-Actin Antibody (M008) [NBP3-23324] -

Immunocytochemistry/Immunofluorescence: beta-Actin Antibody (M008) [NBP3-23324] - Immunocytochemical labeling of beta-Actin in paraformaldehyde fixed human MeWo cells. The cells were labeled with mouse monoclonal anti-beta-Actin (clone M008). The antibody was detected using goat anti-mouse DyLight (R) 594.

Applications for beta-Actin Antibody (M008)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50

Immunoprecipitation

1:100

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: beta-Actin

Actin is a highly conserved protein involved in cell motility, structure, and integrity. Six functional actin isoforms exist and were named based on their electrophoretic mobility and distribution including alpha-cardiac muscle actin (aCAA), alpha-skeletal muscle actin (aSKA), alpha-smooth muscle actin (aSMA), beta-cytoplasmic actin (bCYA), gamma-cytoplasmic actin (gCYA) and gamma-smooth muscle actin (gSMA) (1). The cytoplasmic isoforms, beta-actin and gamma-actin, are nearly identical, differing by only 4 amino acids at the N-terminus with a theoretical molecular weight of 42 kDa (2). Beta-actin is ubiquitously expressed in eukaryotic cells and considered a housekeeping gene which is frequently used as a loading control for assays involving protein detection, such as Western blotting (3,4).

References

1. Vandekerckhove J, Weber K. 1978. At least six different actins are expressed in a higher mammal: an analysis based on the amino acid sequence of the amino-terminal tryptic peptide. J Mol Biol. 126(4):783-802. PMID: 745245

2. Gimona M, Vandekerckhove J, Goethals M, Herzog M, Lando Z, Small JV. (1994) Beta-actin specific monoclonal antibody. Cell Motil Cytoskeleton. 27(2):108-16. PMID: 8162619

3. Holden VI, Lenio S, Kuick R, Ramakrishnan SK, Shah YM, Bachman MA. (2014) Bacterial siderophores that evade or overwhelm Lipocalin 2 induce HIF-1a and pro-inflammatory cytokine secretion in cultured respiratory epithelial cells Infect Immun. 82(9):3826-36 PMID: 24980968

4. Tsai WL, Yeh PH, Tsai CY, Ting CT, Chiu YH, Tao MH, Li WC, Hung SC. (2016) Efficient Programming of Human Mesenchymal Stem Cell Derived Hepatocytes by Epigenetic Regulations. J. Gastroenterol. Hepatol. 32(1):261-269. PMID: 27218433

Alternate Names

ACTB, betaActin

Gene Symbol

ACTB

Additional beta-Actin Products

Product Documents for beta-Actin Antibody (M008)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for beta-Actin Antibody (M008)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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