beta-Actin Antibody [DyLight 680]
Novus Biologicals, part of Bio-Techne | Catalog # NB600-532FR
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Predicted:
Bovine (100%), Canine (100%), Chicken (100%), Chimpanzee (100%), Golden Syrian Hamster (100%), Guinea Pig (100%), Orangutan (100%), Porcine (100%), Rabbit (100%), Rat (100%), Sheep (100%), Xenopus (100%). Backed by our 100% Guarantee.
Applications
ICC/IF (Negative), Immunoprecipitation (Negative)
Label
DyLight 680 (Excitation = 692 nm, Emission = 712 nm)
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This beta-Actin Antibody maps to a region corresponding to the N-terminus of human Beta Actin. [UniProt# P60709]
Reactivity Notes
Based on 100% sequence identity, this antibody is predicted to react with Rat, X. tropicalis, Chicken, Sheep, Bovine, Dog, Horse, Rabbit, Guinea pig, Pig, Golden hamster, Orangutan, and Chimpanzee.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Applications for beta-Actin Antibody [DyLight 680]
Application
Recommended Usage
ICC/IF (Negative)
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation (Negative)
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: beta-Actin
References
1. Vandekerckhove J, Weber K. 1978. At least six different actins are expressed in a higher mammal: an analysis based on the amino acid sequence of the amino-terminal tryptic peptide. J Mol Biol. 126(4):783-802. PMID: 745245
2. Gimona M, Vandekerckhove J, Goethals M, Herzog M, Lando Z, Small JV. (1994) Beta-actin specific monoclonal antibody. Cell Motil Cytoskeleton. 27(2):108-16. PMID: 8162619
3. Holden VI, Lenio S, Kuick R, Ramakrishnan SK, Shah YM, Bachman MA. (2014) Bacterial siderophores that evade or overwhelm Lipocalin 2 induce HIF-1a and pro-inflammatory cytokine secretion in cultured respiratory epithelial cells Infect Immun. 82(9):3826-36 PMID: 24980968
4. Tsai WL, Yeh PH, Tsai CY, Ting CT, Chiu YH, Tao MH, Li WC, Hung SC. (2016) Efficient Programming of Human Mesenchymal Stem Cell Derived Hepatocytes by Epigenetic Regulations. J. Gastroenterol. Hepatol. 32(1):261-269. PMID: 27218433
Alternate Names
ACTB, betaActin
Gene Symbol
ACTB
Additional beta-Actin Products
Product Documents for beta-Actin Antibody [DyLight 680]
Product Specific Notices for beta-Actin Antibody [DyLight 680]
DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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