Key Product Details

Species Reactivity

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # AB9

Concentration

1 mg/ml

View all Amyloid beta Antibodies »

Product Specifications

Immunogen

This beta Amyloid antibody was developed against amino acids 1-16 of human amyloid Abeta.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for beta Amyloid Antibody (AB9)

Immunohistochemistry: beta Amyloid Antibody (AB9) [NBP2-50055]

Immunohistochemistry: beta Amyloid Antibody (AB9) [NBP2-50055]

Immunohistochemistry: beta Amyloid Antibody (AB9) [NBP2-50055] - Analysis of a region of cerebral cortex from an Alzheimer's disease (AD) patient stained with NBP2-50055, the signal detected with HRP and DAB. Senile plaques are labeled SP. The region of the lowest of the three plaques is shown in the inset stained with the fluorescent dye thioflavin-S. This dye binds to not only the senile plaque but also a neurofibrillary tangle (NFT), the other pathological hallmark of AD, which does not contain ABeta.
Western Blot: beta Amyloid Antibody (AB9) [NBP2-50055]

Western Blot: beta Amyloid Antibody (AB9) [NBP2-50055]

Western Blot: beta Amyloid Antibody (AB9) [NBP2-50055] - Amyloid beta peptide blotted with NBP2-50055. NBP2-50055 recognizes amyloid beta peptide running at a molecular weight of 5 kDa and amyloid beta aggregates.

Applications for beta Amyloid Antibody (AB9)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000

Immunohistochemistry

1:1000

Western Blot

1:1000 - 1:2000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50% PBS, 50% glycerol

Preservative

0.035% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Amyloid beta

Beta amyloid, also known as Abeta, is a peptide that varies in length from 39 to 43 amino acids and is processed from the transmembrane protein amyloid precursor protein (APP) by beta-secretase (BACE-1) and gamma-secretase. Beta amyloid [1-40], beta amyloid [1-42], and beta amyloid [1-43] peptides result from cleavage of APP after residues 40, 42, and 43, respectively. The aggregation of beta amyloid monomers produces insoluble oligomers and protofibrils. The longer "stickier" forms of beta amyloid, particularly ABeta42, are known as the main component of amyloid plaques, which are deposits (senile plaques or neuritic plaques) found in the brains of patients with Alzheimer's disease (AD) (1,2). Research indicates that intraneuronal beta amyloid accumulation may be an important proximal neurotoxic event in the pathogenesis of Alzheimer's disease. Abeta deposits have also been reported in the hearts of AD patients (3).

Pyroglutamate amyloid beta peptides (pGlu-Abeta) are N-terminal truncations in which the N-terminal glutamate is cyclized to pyroglutamate resulting in pGlu-Abeta (3-40/42 and 11-40/42) (4). This pyrE modification may have a greater propensity to aggregate under physiological conditions and has been implicated as the molecular species responsible for seeding larger oligomers of amyloid beta.

References

1. Chen GF, Xu TH, Yan Y, Zhou YR, Jiang Y, Melcher K, Xu HE. (2017) Amyloid beta: structure, biology and structure-based therapeutic development. Acta Pharmacol Sin. 38(9):1205-1235. PMID: 28713158

2. De-Paula VJ1, Radanovic M, Diniz BS, Forlenza OV. (2012) Alzheimer's disease. Subcell Biochem. 65:329-52. PMID: 23225010

3. Schaich CL, Maurer MS, Nadkarni NK. (2019) Amyloidosis of the Brain and Heart: Two Sides of the Same Coin? JACC Heart Fail. 7(2):129-131. PMID: 30704604

4. He W, Barrow CJ. (1999) The A beta 3-pyroglutamyl and 11-pyroglutamyl peptides found in senile plaque have greater beta-sheet forming and aggregation propensities in vitro than full-length A beta. Biochemistry. 38(33):10871-7. PMID: 10451383

Alternate Names

Abeta, beta-Amyloid Peptide

Gene Symbol

APP

UniProt

P05067

Additional Amyloid beta Products

Product Documents for beta Amyloid Antibody (AB9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for beta Amyloid Antibody (AB9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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