beta-Arrestin 2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-1515
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-HDHIPLPRPQS corresponding to internal region according to NP_004304.1, NP_945355.1.
Specificity
This antibody is expected to recognise both reported isoforms 1, 2, 3, 5 and 6 (NP_004304.1; NP_945355.1; NP_001244257.1; NP_001244259.1; NP_001244260.1). No crossreactivity is expected with Arrestin beta 1.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for beta-Arrestin 2 Antibody
![Western Blot: beta-Arrestin 2 Antibody [NB100-1515]](https://resources.bio-techne.com/images/products/beta-Arrestin-2-Antibody-Western-Blot-NB100-1515-img0001.jpg "Western Blot: beta-Arrestin 2 Antibody [NB100-1515]")
Western Blot: beta-Arrestin 2 Antibody [NB100-1515]
Western Blot: beta-Arrestin 2 Antibody [NB100-1515] - Staining of A549 (A),and U251 (B) cell lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.![Immunocytochemistry/ Immunofluorescence: beta-Arrestin 2 Antibody [NB100-1515]](https://resources.bio-techne.com/images/products/beta-Arrestin-2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1515-img0002.jpg "Immunocytochemistry/ Immunofluorescence: beta-Arrestin 2 Antibody [NB100-1515]")
Immunocytochemistry/ Immunofluorescence: beta-Arrestin 2 Antibody [NB100-1515]
Immunocytochemistry/Immunofluorescence: beta-Arrestin 2 Antibody [NB100-1515] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing plasma membrane, cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).![Flow Cytometry: beta-Arrestin 2 Antibody [NB100-1515]](https://resources.bio-techne.com/images/products/beta-Arrestin-2-Antibody-Flow-Cytometry-NB100-1515-img0003.jpg "Flow Cytometry: beta-Arrestin 2 Antibody [NB100-1515]")
Flow Cytometry: beta-Arrestin 2 Antibody [NB100-1515]
Flow Cytometry: beta-Arrestin 2 Antibody [NB100-1515] - Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.Applications for beta-Arrestin 2 Antibody
Application
Recommended Usage
Flow Cytometry
10 ug/mL
Immunocytochemistry/ Immunofluorescence
10 ug/mL
Peptide ELISA
Detection limit 1:64000
Western Blot
0.5 - 1 ug/mL
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: beta-Arrestin 2
Alternate Names
ARB2, ARR2, ARRB2, betaArrestin 2
Entrez Gene IDs
409 (Human)
Gene Symbol
ARRB2
UniProt
Additional beta-Arrestin 2 Products
Product Documents for beta-Arrestin 2 Antibody
Product Specific Notices for beta-Arrestin 2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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