Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Chicken, Duck, Primate

Cited:

Human, Mouse, Rat, Avian - Chicken, Avian - Duck

Applications

Validated:

Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot

Cited:

Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 12F7

Format

BSA Free

Concentration

1.0 mg/ml

View all beta-Catenin Antibodies »

Product Specifications

Immunogen

Recombinant chicken beta Catenin fused to maltose binding protein. [UniProt# O42486]

Reactivity Notes

Use in Duck reported in scientific literature (PMID:32692763).

Localization

Nucleus, Cytoplasm, Cytoskeleton, Adherens junction, Centrosome, Spindle pole.

Marker

Epithelial Cell Marker, Adherens Junction Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

92 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for beta-Catenin Antibody (12F7) - BSA Free

Western Blot: beta-Catenin Antibody (12F7)BSA Free [NBP1-54467]

Western Blot: beta-Catenin Antibody (12F7)BSA Free [NBP1-54467]

Western Blot: beta-Catenin Antibody (12F7) [NBP1-54467] - Total protein from human HepG2 and HeLa cells, rat PC12 cells and mouse 3T3 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Beta catenin in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Western Blot: beta-Catenin Antibody (12F7)BSA Free [NBP1-54467]

Western Blot: beta-Catenin Antibody (12F7)BSA Free [NBP1-54467]

Western Blot: beta-Catenin Antibody (12F7) [NBP1-54467] - Activity of Wnt/beta-catenin pathway in the embryonic chick lung. Western blot analysis of active and total beta-catenin in stage b1, b2 and b3 lungs, and stage 24 limb (as positive control). Control loading was performed using beta-tubulin (55 kDa). Total and active beta-catenin correspond to 92 kDa. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0112388), licensed under a CC-BY license.
Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody (12F7) - BSA Free [NBP1-54467]

Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody (12F7) - BSA Free [NBP1-54467]

Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody (12F7) [NBP1-54467] - The beta- Catenin antibody was tested in MCF-7 cells against Dylight 488 (Green). Actin and nuclei were counterstained against Phalloidin 550 (Red) and DAPI (Blue).
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Applications for beta-Catenin Antibody (12F7) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Immunohistochemistry

1:100-1:200

Immunohistochemistry-Paraffin

1:100-1:200

Immunoprecipitation

1:10-1:500

Simple Western

1:100

Western Blot

1:1000
Application Notes
This beta Catenin (12F7) antibody is useful for IHC-P sections, ICC/IF, IP and WB, where a band can be seen at approx. 92 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 4 reviews rated 4 using NBP1-54467 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: beta-Catenin

The beta-catenin protein functions in both regulation and coordination of cell-cell adhesion and gene transcription. In humans, beta-catenin the CTNNB1 gene. beta-catenin is a subunit of the cadherin protein complex and acts as an intracellular signal transducer in the Wnt signaling pathway. It is a member of the catenin protein family and homologous to gamma-catenin, also known as plakoglobin. Beta-catenin is widely expressed in many tissues. In cardiac muscle, beta-catenin localizes to adherens junctions in intercalated disc structures, which are critical for electrical and mechanical coupling between adjacent cardiomyocytes.

Alternate Names

bCatenin, CTNNB1

Entrez Gene IDs

1499 (Human); 12387 (Mouse); 84353 (Rat); 395964 (Chicken)

Gene Symbol

CTNNB1

UniProt

P35222, O42486 (Chicken), Q02248 (Mouse), Q9WU82 (Rat)

Additional beta-Catenin Products

Product Documents for beta-Catenin Antibody (12F7) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for beta-Catenin Antibody (12F7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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