beta-Catenin Antibody (CTNNB1/2030R) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-79888
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # CTNNB1/2030R
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant full-length human beta-Catenin protein (Uniprot: P35222 )
Localization
Cell Surface, Cytoplasm
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Theoretical MW
92 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified by Protein A Column. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-79753).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for beta-Catenin Antibody (CTNNB1/2030R) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody (CTNNB1/2030R) - Azide and BSA Free [NBP2-79888]
Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody (CTNNB1/2030R) - Azide and BSA Free [NBP2-79888] - Immunofluorescence Analysis of PFA fixed HeLa cells labeling Beta-Catenin with Beta-Catenin Recombinant Rabbit Monoclonal Ab (CTNNB1/2030R followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).Immunohistochemistry-Paraffin: beta-Catenin Antibody (CTNNB1/2030R) - Azide and BSA Free [NBP2-79888]
Immunohistochemistry-Paraffin: beta-Catenin Antibody (CTNNB1/2030R) - Azide and BSA Free [NBP2-79888] - Formalin-fixed, paraffin-embedded human Cervical Carcinoma stained with Beta-Catenin Recombinant Rabbit Monoclonal Antibody (CTNNB1/2030R).Flow Cytometry: beta-Catenin Antibody (CTNNB1/2030R) - Azide and BSA Free [NBP2-79888]
Flow Cytometry: beta-Catenin Antibody (CTNNB1/2030R) - Azide and BSA Free [NBP2-79888] - Flow Cytometric Analysis of PFA fixed HeLa cells using Beta-Catenin Recombinant Rabbit Monoclonal Ab (CTNNB1/2030R) followed by Goat anti-Rabbit IgG-CF488 (Blue); Isotype Control (Red).Applications for beta-Catenin Antibody (CTNNB1/2030R) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: beta-Catenin
Alternate Names
bCatenin, CTNNB1
Gene Symbol
CTNNB1
Additional beta-Catenin Products
Product Documents for beta-Catenin Antibody (CTNNB1/2030R) - Azide and BSA Free
Product Specific Notices for beta-Catenin Antibody (CTNNB1/2030R) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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