Beta Dystroglycan Antibody (GT835)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13643

Novus Biologicals, part of Bio-Techne
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NBP3-13643
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # GT835

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Beta Dystroglycan Antibodies »

Product Summary for Beta Dystroglycan Antibody (GT835)

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Beta Dystroglycan / beta Dystroglycan. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

97 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for Beta Dystroglycan Antibody (GT835)

Western Blot: Beta Dystroglycan Antibody (GT835) [NBP3-13643]

Western Blot: Beta Dystroglycan Antibody (GT835) [NBP3-13643]

Western Blot: Beta Dystroglycan Antibody (GT835) [NBP3-13643] - Wild-type (WT) and Beta Dystroglycan knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Beta Dystroglycan antibody [GT835] (NBP3-13643) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: Beta Dystroglycan Antibody (GT835) [NBP3-13643]

Immunocytochemistry/ Immunofluorescence: Beta Dystroglycan Antibody (GT835) [NBP3-13643]

Immunocytochemistry/Immunofluorescence: Beta Dystroglycan Antibody (GT835) [NBP3-13643] - Beta Dystroglycan antibody [GT835] detects Beta Dystroglycan protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Beta Dystroglycan stained by Beta Dystroglycan antibody [GT835] (NBP3-13643) diluted at 1:100. Blue: Fluoroshield with DAPI. Scale bar= 10 um.
Immunohistochemistry-Paraffin: Beta Dystroglycan Antibody (GT835) [NBP3-13643]

Immunohistochemistry-Paraffin: Beta Dystroglycan Antibody (GT835) [NBP3-13643]

Immunohistochemistry-Paraffin: Beta Dystroglycan Antibody (GT835) [NBP3-13643] - Mouse placenta. Beta Dystroglycan stained by Beta Dystroglycan antibody [835] (NBP3-13643) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
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Applications for Beta Dystroglycan Antibody (GT835)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Assay dependent

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Beta Dystroglycan

The dystroglycan complex is a membrane-spanning complex composed of two subunits, alpha- and beta-dystroglycan. Alpha-dystroglycan is a cell surface peripheral membrane protein which binds to the extracellular matrix (ECM), whereas beta-dystroglycan is an integral membrane protein which anchors alpha-dystroglycan to the cell membrane. The dystroglycan complex provides a tight link between the ECM and cell membrane. Dysfunction of the dystroglycan complex has commonly been implicated in the molecular pathogenesis of severe forms of hereditary neuromuscular diseases, including Duchenne muscular dystrophy, Fukuyama-type congenital muscular dystrophy and sarcoglycanopathy (1). Human dystroglycan is expressed in a variety of fetal and adult tissues. Data suggest that muscle and non-muscle isoforms of dystroglycan differ by carbohydrate moieties but not protein sequence. Therefore variable glycosylation of the conserved protein core might modulate laminin binding (2). beta-dystroglycan has been localized to microvilli structures in a number of cell types where it associates with the cytoskeletal adaptor ezrin, through which it is able to modulate the actin cytoskeleton and induce peripheral filopodia and microvilli (3).

Alternate Names

A3a, AGRNR, DAG156DAG, dystroglycan, dystroglycan 1 (dystrophin-associated glycoprotein 1), Dystrophin-associated glycoprotein 1

Gene Symbol

DAG1

Additional Beta Dystroglycan Products

Product Documents for Beta Dystroglycan Antibody (GT835)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Beta Dystroglycan Antibody (GT835)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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