beta-Galactosidase-1/GLB1 Antibody (JE40-77)

Western Blot: beta-Galactosidase-1/GLB1 Antibody (JE40-77) [NBP3-32383]

Western Blot: beta-Galactosidase-1/GLB1 Antibody (JE40-77) [NBP3-32383] - Western blot analysis of beta-Galactosidase-1/GLB1 on different lysates with Rabbit anti-beta-Galactosidase-1/GLB1 antibody (NBP3-32383) at 1/1,000 dilution.

Lane 1: SK-Br-3 cell lysate (20 ug/Lane)
Lane 2: Saos-2 cell lysate (20 ug/Lane)
Lane 3: U-87 MG cell lysate (20 ug/Lane)
Lane 4: MCF7 cell lysate (20 ug/Lane)
Lane 5: HepG2 cell lysate (20 ug/Lane)
Lane 6: K-562 cell lysate (20 ug/Lane)
Lane 7: Daudi cell lysate (20 ug/Lane)
Lane 8: NIH/3T3 cell lysate (20 ug/Lane)
Lane 9: Rat brain tissue lysate (40 ug/Lane)

Predicted band size: 76 kDa
Observed band size: 76/100 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32383) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Flow Cytometry: beta-Galactosidase-1/GLB1 Antibody (JE40-77) [NBP3-32383]

Flow Cytometry: beta-Galactosidase-1/GLB1 Antibody (JE40-77) [NBP3-32383] - Flow cytometric analysis of SK-Br-3 cells labeling beta-Galactosidase-1/GLB1.

Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32383, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).