beta Galactosidase Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB600-305
![Western Blot: beta Galactosidase Antibody [NB600-305]](https://resources.bio-techne.com/images/products/beta-Galactosidase-Antibody-Western-Blot-NB600-305-img0016.jpg "Western Blot: beta Galactosidase Antibody [NB600-305]")
![Western Blot: beta Galactosidase Antibody [NB600-305]](https://resources.bio-techne.com/images/products/beta-Galactosidase-Antibody-Western-Blot-NB600-305-img0017.jpg "Western Blot: beta Galactosidase Antibody [NB600-305]")
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Full length native beta Galactosidase isolated from E.coli
Reactivity Notes
Cross reactivity against beta Galactosidase from other tissues and species may occur but have not been specifically determined
Localization
Cytoplasmic
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Beta-Galactosidase Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Beta Galactosidase [E.coli]
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for beta Galactosidase Antibody
Western Blot: beta Galactosidase Antibody [NB600-305]
Western Blot: beta Galactosidase Antibody [NB600-305] - Analysis using FITC conjugate of NB600-305. 3) corresponding to 60 ng, 30 ng and 15 ng, respectively of b-Gal present in partially purified preparations (arrowhead). Lane 4 shows no cross reactivity with proteins present in a non-specific control E.coli lysate. Proteins were resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred to nitrocellulose and blocking using Blocking Buffer for Fluorescent Western Blotting. The membrane was probed with fluorescein conjugated anti-b-Galactosidase diluted to 1:10,000. Reaction occurred for 2 hours at room temperature.![Western Blot: beta Galactosidase Antibody [NB600-305]](https://resources.bio-techne.com/images/products/beta-Galactosidase-Antibody-Western-Blot-NB600-305-img0014.jpg "Western Blot: beta Galactosidase Antibody [NB600-305]")
Western Blot: beta Galactosidase Antibody [NB600-305]
Western Blot: beta Galactosidase Antibody [NB600-305] - Analysis of a band at 117 kDa (lane 1) corresponding to b-Gal present in a partially purified preparation (arrowhead). Approximately 1 ug of protein was resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking, the membrane was probed with the primary antibody diluted to 1:1,000. Reaction occurred overnight at 4 C followed by washes and reaction with a 1:10,000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 for 45 min at room temperature (800 nm channel, green).![Western Blot: beta Galactosidase Antibody [NB600-305]](https://resources.bio-techne.com/images/products/beta-Galactosidase-Antibody-Western-Blot-NB600-305-img0015.jpg "Western Blot: beta Galactosidase Antibody [NB600-305]")
Western Blot: beta Galactosidase Antibody [NB600-305]
Western Blot: beta Galactosidase Antibody [NB600-305] - Lane 1 shows 80 ng and lane 2 shows 20 ng loaded onto gel. Results for non-reducing conditions of SDS-PAGE prior to transfer to nitrocellulose are shown on the left side of the figure; results obtainined under reducing conditions are shown on the right. Blots were blocked overnight at 4 C with Blocking Buffer for Fluorescent Western Blotting. The membrane was probed with anti-b-Galactosidase diluted to 1:10,000. Reaction occurred overnight at 4C. Dylight649 conjugated Gt-a-anti-Rabbit IgG was used for detection.
Applications for beta Galactosidase Antibody
Application
Recommended Usage
ELISA
1:10000
Immunocytochemistry/ Immunofluorescence
1:10 - 1:500
Immunohistochemistry
1:5000
Immunohistochemistry-Frozen
1:1500
Immunohistochemistry-Paraffin
1:10 - 1:500
Western Blot
1:5000-1:10000
Application Notes
This product has been tested by ELISA and western blot and is suitable for dot blot, immunofluorescence microscopy, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity. The antibody recognizes both frozen tissue sections, paraffin embedded tissue and 4% paraformaldehyde fixed tissue for most immunohistochemical analysis. A 1:1,500 dilution has been reported to detect beta-galactosidase in adult rat spinal cord tissue after infection with helper-dependent adenovirus expressing lacZ. In this particular experiment, tissue was perfused with 4% paraformaldehyde and cryostat-cut (35 um) to produce free-floating sections.br/>
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 23838935).
Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 25799059)
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 23838935).
Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 25799059)
Reviewed Applications
Read 1 review rated 5 using NB600-305 in the following applications:
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 100 ul deionized water (or equivalent)
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: beta Galactosidase
Alternate Names
EC 3.2.1.23; b-gal
UniProt
Additional beta Galactosidase Products
Product Documents for beta Galactosidase Antibody
Product Specific Notices for beta Galactosidase Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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