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Beta Hydroxysteroid Dehydrogenase Antibody

Novus Biologicals, part of Bio-Techne | Catalog # H00003284-D01P

Novus Biologicals, part of Bio-Techne
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H00003284-D01P

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

HSD3B2 (NP_000189.1, 1 a.a. - 372 a.a.) full-length human protein. MGWSCLVTGAGGLLGQRIVRLLVEEKELKEIRALDKAFRPELREEFSKLQNRTKLTVLEGDILDEPFLKRACQDVSVVIHTACIIDVFGVTHRESIMNVNVKGTQLLLEACVQASVPVFIYTSSIEVAGPNSYKEIIQNGHEEEPLENTWPTPYPYSKKLAEKAVLAANGWNLKNGDTLYTCALRPTYIYGEGGPFLSASINEALNNNGILSSVGKFSTVNPVYVGNVAWAHILALRALRDPKKAPSVRGQFYYISDDTPHQSYDNLNYILSKEFGLRLDSRWSLPLTLMYWIGFLLEVVSFLLSPIYSYQPPFNRHTVTLSNSVFTFSYKKAQRDLAYKPLYSWEEAKQKTVEWVGSLVDRHKETLKSKTQ

Specificity

Reacts with hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Quality control test: Antibody reactive against mammalian transfected lysate.

Scientific Data Images for Beta Hydroxysteroid Dehydrogenase Antibody

Western Blot: Beta Hydroxysteroid Dehydrogenase Antibody [H00003284-D01P]

Western Blot: Beta Hydroxysteroid Dehydrogenase Antibody [H00003284-D01P]

Western Blot: Beta Hydroxysteroid Dehydrogenase Antibody [H00003284-D01P] - Analysis of HSD3B2 expression in human placenta.
Western Blot: Beta Hydroxysteroid Dehydrogenase Antibody [H00003284-D01P]

Western Blot: Beta Hydroxysteroid Dehydrogenase Antibody [H00003284-D01P]

Western Blot: Beta Hydroxysteroid Dehydrogenase Antibody [H00003284-D01P] - Analysis of HSD3B2 expression in transfected 293T cell line by HSD3B2 polyclonal antibody.Lane 1: HSD3B2 transfected lysate(42.10 KDa).Lane 2: Non-transfected lysate.

Applications for Beta Hydroxysteroid Dehydrogenase Antibody

Application
Recommended Usage

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is reactive against tissue and transfected lysate in WB and as a detection antibody in ELISA.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Beta Hydroxysteroid Dehydrogenase

The protein encoded by the Beta Hydroxysteroid Dehydrogenases gene is a bifunctional enzyme that catalyzes the oxidative conversion ofdelta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. It plays a crucial role in thebiosynthesis of all classes of hormonal steroids. This gene is predominantly expressed in the adrenals and the gonads.Mutations in this gene are associated with 3-beta-hydroxysteroid dehydrogenase, type II, deficiency. Alternativelyspliced transcript variants have been found for this gene. (provided by RefSeq)

Alternate Names

3 beta-HSD type II, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type II, 3-beta-HSD II, delta 5-delta 4-isomerase type II, HSD3B, HSDB, HSDB3B, hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2,3-beta-hydroxy-Delta(5)-steroid dehydrogenase, progesterone reductase, SDR11E2, short chain dehydrogenase/reductase family 11E, member 2,3-beta-hydroxy-5-ene steroid dehydrogenase

Entrez Gene IDs

3284 (Human)

Gene Symbol

HSD3B2

Additional Beta Hydroxysteroid Dehydrogenase Products

Product Documents for Beta Hydroxysteroid Dehydrogenase Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Beta Hydroxysteroid Dehydrogenase Antibody

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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