Beta Hydroxysteroid Dehydrogenase Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-92640

Novus Biologicals, part of Bio-Techne
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NBP2-92640-0.02ml
NBP2-92640-0.1ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Beta Hydroxysteroid Dehydrogenase Antibodies »

Product Summary for Beta Hydroxysteroid Dehydrogenase Antibody - Azide and BSA Free

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-287 of human HSD3B2 (NP_000189.1). MGWSCLVTGAGGLLGQRIVRLLVEEKELKEIRALDKAFRPELREEFSKLQNRTKLTVLEGDILDEPFLKRACQDVSVVIHTACIIDVFGVTHRESIMNVNVKGTQLLLEACVQASVPVFIYTSSIEVAGPNSYKEIIQNGHEEEPLENTWPTPYPYSKKLAEKAVLAANGWNLKNGDTLYTCALRPTYIYGEGGPFLSASINEALNNNGILSSVGKFSTVNPVYVGNVAWAHILALRALRDPKKAPSVRGQFYYISDDTPHQSYDNLNYILSKEFGLRLDSRWSLPL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Beta Hydroxysteroid Dehydrogenase Antibody - Azide and BSA Free

Western Blot: Beta Hydroxysteroid Dehydrogenase AntibodyAzide and BSA Free [NBP2-92640]

Western Blot: Beta Hydroxysteroid Dehydrogenase AntibodyAzide and BSA Free [NBP2-92640]

Western Blot: Beta Hydroxysteroid Dehydrogenase Antibody [NBP2-92640] - Analysis of extracts of various cell lines, using Beta Hydroxysteroid Dehydrogenase at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunocytochemistry/ Immunofluorescence: Beta Hydroxysteroid Dehydrogenase Antibody - Azide and BSA Free [NBP2-92640]

Immunocytochemistry/ Immunofluorescence: Beta Hydroxysteroid Dehydrogenase Antibody - Azide and BSA Free [NBP2-92640]

Immunocytochemistry/Immunofluorescence: Beta Hydroxysteroid Dehydrogenase Antibody [NBP2-92640] - Analysis of A-549 cells using Beta Hydroxysteroid Dehydrogenase .

Applications for Beta Hydroxysteroid Dehydrogenase Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:100

Western Blot

1:100 - 1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

Azide and BSA Free

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Beta Hydroxysteroid Dehydrogenase

The protein encoded by the Beta Hydroxysteroid Dehydrogenases gene is a bifunctional enzyme that catalyzes the oxidative conversion ofdelta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. It plays a crucial role in thebiosynthesis of all classes of hormonal steroids. This gene is predominantly expressed in the adrenals and the gonads.Mutations in this gene are associated with 3-beta-hydroxysteroid dehydrogenase, type II, deficiency. Alternativelyspliced transcript variants have been found for this gene. (provided by RefSeq)

Alternate Names

3 beta-HSD type II, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type II, 3-beta-HSD II, delta 5-delta 4-isomerase type II, HSD3B, HSDB, HSDB3B, hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2,3-beta-hydroxy-Delta(5)-steroid dehydrogenase, progesterone reductase, SDR11E2, short chain dehydrogenase/reductase family 11E, member 2,3-beta-hydroxy-5-ene steroid dehydrogenase

Gene Symbol

HSD3B2

Additional Beta Hydroxysteroid Dehydrogenase Products

Product Documents for Beta Hydroxysteroid Dehydrogenase Antibody - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Beta Hydroxysteroid Dehydrogenase Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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