beta I Tubulin Antibody (16E2)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26267
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Flow Cytometry, Immunohistochemistry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG Clone # 16E2
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for beta I Tubulin Antibody (16E2)
Immunogen
Recombinant Human beta I Tubulin protein [UniProt Q9H4B7]
Clonality
Monoclonal
Host
Mouse
Isotype
IgG
Scientific Data Images for beta I Tubulin Antibody (16E2)
Immunohistochemistry: beta I Tubulin Antibody (16E2) [NBP3-26267] -
Immunohistochemistry: beta I Tubulin Antibody (16E2) [NBP3-26267] - Image of beta I Tubulin Antibody (16E2) diluted at 1:300 and staining in paraffin-embedded human pancreatic cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
Immunohistochemistry: beta I Tubulin Antibody (16E2) [NBP3-26267] -
Immunohistochemistry: beta I Tubulin Antibody (16E2) [NBP3-26267] - Image of beta I Tubulin Antibody (16E2) diluted at 1:300 and staining in paraffin-embedded human pancreatic cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
Western Blot: beta I Tubulin Antibody (16E2) [NBP3-26267] -
Western Blot: beta I Tubulin Antibody (16E2) [NBP3-26267] - Positive Western Blot detected in: K562 whole cell lysate.All lanes: beta I Tubulin Antibody at 1: 1000
Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution.
Predicted band size: 50 kDa
Observed band size: 50 kDa
Applications for beta I Tubulin Antibody (16E2)
Application
Recommended Usage
Flow Cytometry
1:50-1:200
Immunohistochemistry
1:50-1:200
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
50% Glycerol, 0.01M PBS, PH 7.4
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: beta-I Tubulin
Long Name
Tubulin, Beta 1 Class VI
Alternate Names
betaI Tubulin, TUBB1
Gene Symbol
TUBB1
Additional beta-I Tubulin Products
Product Documents for beta I Tubulin Antibody (16E2)
Product Specific Notices for beta I Tubulin Antibody (16E2)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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