beta I Tubulin Antibody (OTI4A3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-46245

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Monkey

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI4A3

Concentration

1 mg/ml

View all beta-I Tubulin Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human TUBB1(NP_110400) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

50.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for beta I Tubulin Antibody (OTI4A3)

Western Blot: beta I Tubulin Antibody (OTI4A3) [NBP2-46245]

Western Blot: beta I Tubulin Antibody (OTI4A3) [NBP2-46245]

Western Blot: beta I Tubulin Antibody (OTI4A3) [NBP2-46245] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY beta 1 Tubulin.
Immunocytochemistry/ Immunofluorescence: beta I Tubulin Antibody (OTI4A3) [NBP2-46245]

Immunocytochemistry/ Immunofluorescence: beta I Tubulin Antibody (OTI4A3) [NBP2-46245]

Immunocytochemistry/Immunofluorescence: beta I Tubulin Antibody (OTI4A3) [NBP2-46245] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY beta I Tubulin.
Immunohistochemistry: beta I Tubulin Antibody (OTI4A3) [NBP2-46245]

Immunohistochemistry: beta I Tubulin Antibody (OTI4A3) [NBP2-46245]

Immunohistochemistry: beta I Tubulin Antibody (OTI4A3) [NBP2-46245] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Applications for beta I Tubulin Antibody (OTI4A3)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:200-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: beta-I Tubulin

Microtubules are involved in a wide variety of cellular processes, including mitosis, morphogenesis, plateletformation, and mobility of cilia and flagella. Circulating platelets carry a single marginal microtubule coil that iswound in 8 to 12 turns and is responsible for platelet shape. TUBB1 is the major beta-tubulin expressed in plateletsand megakaryocytes and is required for optimal platelet assembly (Wang et al., 1986 (PubMed 3782288); Schulze et al.,2004 (PubMed 15315966)).(supplied by OMIM)

Long Name

Tubulin, Beta 1 Class VI

Alternate Names

betaI Tubulin, TUBB1

Gene Symbol

TUBB1

Additional beta-I Tubulin Products

Product Documents for beta I Tubulin Antibody (OTI4A3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for beta I Tubulin Antibody (OTI4A3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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