Beta Ig-h3/TGFBI Antibody (7Q5O9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15994

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15994-100ul
NBP3-15994-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7Q5O9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all beta IG-H3 Antibodies »

Product Summary for Beta Ig-h3/TGFBI Antibody (7Q5O9)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 50-150 of human Beta Ig-h3/TGFBI (Q15582). AVQKVIGTNRKYFTNCKQWYQRKICGKSTVISYECCPGYEKVPGEKGCPAALPLSNLYETLGVVGSTTTQLYTDRTEKLRPEMEGPGSFTIFAPSNEAWAS

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Beta Ig-h3/TGFBI Antibody (7Q5O9)

Western Blot: Beta Ig-h3/TGFBI Antibody (7Q5O9) [NBP3-15994]

Western Blot: Beta Ig-h3/TGFBI Antibody (7Q5O9) [NBP3-15994]

Western Blot: Beta Ig-h3/TGFBI Antibody (7Q5O9) [NBP3-15994] - Analysis of extracts of various cell lines, using TGF beta induced Beta Ig-h3/TGFBI Rabbit mAb (NBP3-15994) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody (7Q5O9) [NBP3-15994]

Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody (7Q5O9) [NBP3-15994]

Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody (7Q5O9) [NBP3-15994] - Human esophageal using TGF beta induced Beta Ig-h3/TGFBI Rabbit mAb (NBP3-15994) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Western Blot: Beta Ig-h3/TGFBI Antibody (7Q5O9) [NBP3-15994]

Western Blot: Beta Ig-h3/TGFBI Antibody (7Q5O9) [NBP3-15994]

Western Blot: Beta Ig-h3/TGFBI Antibody (7Q5O9) [NBP3-15994] - Analysis of extracts of various cell lines, using TGF beta induced Beta Ig-h3/TGFBI Rabbit mAb (NBP3-15994) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Applications for Beta Ig-h3/TGFBI Antibody (7Q5O9)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: beta IG-H3

TGFBI encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion. Mutations in this gene are associated with multiple types of corneal dystrophy. [provided by RefSeq]

Long Name

TGF-beta Induced Gene H3

Alternate Names

beta IGH3, Kerato-epithelin, RGD-CAP, TGFBI, TGFBIP

Gene Symbol

TGFBI

Additional beta IG-H3 Products

Product Documents for Beta Ig-h3/TGFBI Antibody (7Q5O9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Beta Ig-h3/TGFBI Antibody (7Q5O9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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