Beta Ig-h3/TGFBI Antibody (7Q5O9)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15994
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 7Q5O9
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human Beta Ig-h3/TGFBI (Q15582). AVQKVIGTNRKYFTNCKQWYQRKICGKSTVISYECCPGYEKVPGEKGCPAALPLSNLYETLGVVGSTTTQLYTDRTEKLRPEMEGPGSFTIFAPSNEAWAS
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Beta Ig-h3/TGFBI Antibody (7Q5O9)
Western Blot: Beta Ig-h3/TGFBI Antibody (7Q5O9) [NBP3-15994]
Western Blot: Beta Ig-h3/TGFBI Antibody (7Q5O9) [NBP3-15994] - Analysis of extracts of various cell lines, using TGF beta induced Beta Ig-h3/TGFBI Rabbit mAb (NBP3-15994) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody (7Q5O9) [NBP3-15994]
Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody (7Q5O9) [NBP3-15994] - Human esophageal using TGF beta induced Beta Ig-h3/TGFBI Rabbit mAb (NBP3-15994) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.Western Blot: Beta Ig-h3/TGFBI Antibody (7Q5O9) [NBP3-15994]
Western Blot: Beta Ig-h3/TGFBI Antibody (7Q5O9) [NBP3-15994] - Analysis of extracts of various cell lines, using TGF beta induced Beta Ig-h3/TGFBI Rabbit mAb (NBP3-15994) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.Applications for Beta Ig-h3/TGFBI Antibody (7Q5O9)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: beta IG-H3
Long Name
TGF-beta Induced Gene H3
Alternate Names
beta IGH3, Kerato-epithelin, RGD-CAP, TGFBI, TGFBIP
Gene Symbol
TGFBI
Additional beta IG-H3 Products
Product Documents for Beta Ig-h3/TGFBI Antibody (7Q5O9)
Product Specific Notices for Beta Ig-h3/TGFBI Antibody (7Q5O9)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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