Beta Ig-h3/TGFBI Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-47215

Novus Biologicals, part of Bio-Techne
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NBP1-47215
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Rat

Predicted:

Canine (100%), Mouse (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Cited:

Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all beta IG-H3 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-QLYTDRTEKLRPE corresponding to internal region according to NP_000349.1.

Epitope

Internal region

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Beta Ig-h3/TGFBI Antibody

Western Blot: Beta Ig-h3/TGFBI Antibody [NBP1-47215]

Western Blot: Beta Ig-h3/TGFBI Antibody [NBP1-47215]

Western Blot: Beta Ig-h3/TGFBI Antibody [NBP1-47215] - Staining of Human Kidney (A), Colorectal cancer (B) cell lysate with antibody at 0.3 ug/mL, Human Prostate (C) and Rat Kidney (D) cell lysate with antibody at 0.1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: Beta Ig-h3/TGFBI Antibody [NBP1-47215]

Immunocytochemistry/ Immunofluorescence: Beta Ig-h3/TGFBI Antibody [NBP1-47215]

Immunocytochemistry/Immunofluorescence: Beta Ig-h3/TGFBI Antibody [NBP1-47215] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing golgi and membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody [NBP1-47215]

Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody [NBP1-47215]

Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody [NBP1-47215] - Staining of paraffin embedded human kidney. Antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for Beta Ig-h3/TGFBI Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Immunohistochemistry

4 - 6 ug/mL

Immunohistochemistry-Paraffin

4 - 6 ug/mL

Peptide ELISA

Detection limit 1:32000

Western Blot

0.1 - 0.3 ug/mL
Application Notes
Use in Immunohistochemistry-Frozen reported in scientific literature (PMID:35063020)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: beta IG-H3

TGFBI, also known as BIGH3 and betaig-h3, is an extracellular matrix protein induced by transforming growth factor (TGF)-beta 1. TGFBI protein is involved in cell growth, cell differentiation, wound healing and cell adhesion. In addition, some missense mutations of TGFBI were identified in families affected with human autosomal dominant corneal dystrophies. BIGH3 gene encodes for a 683 amino-acid protein containing an RGD motif and four internal repeated domains which have highly conserved sequences founded in several species (Fasciclin domain). Clone k1H12, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human TGFBI protein.

Long Name

TGF-beta Induced Gene H3

Alternate Names

beta IGH3, Kerato-epithelin, RGD-CAP, TGFBI, TGFBIP

Entrez Gene IDs

7045 (Human); 21810 (Mouse); 24511 (Rat)

Gene Symbol

TGFBI

UniProt

NP_000349.1 (Human)

Additional beta IG-H3 Products

Product Documents for Beta Ig-h3/TGFBI Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Beta Ig-h3/TGFBI Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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