beta-III Tubulin Antibody (CL5814)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61431

Novus Biologicals, part of Bio-Techne
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NBP2-61431
NBP2-61431-25ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL5814

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all beta-III Tubulin Antibodies »

Product Specifications

Immunogen

This antibody was developed against a synthetic peptide corresponding to amino acids: YEDDEEESEAQGPK

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for beta-III Tubulin Antibody (CL5814)

Western Blot: beta-III Tubulin Antibody (CL5814) [NBP2-61431]

Western Blot: beta-III Tubulin Antibody (CL5814) [NBP2-61431]

Western Blot: beta-III Tubulin Antibody (CL5814) [NBP2-61431] - Analysis in human cerebral cortex tissue.
Immunocytochemistry/ Immunofluorescence: beta-III Tubulin Antibody (CL5814) [NBP2-61431]

Immunocytochemistry/ Immunofluorescence: beta-III Tubulin Antibody (CL5814) [NBP2-61431]

Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody (CL5814) [NBP2-61431] - Staining of A549 cells. Nuclear probes are visualized in blue. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5814) [NBP2-61431]

Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5814) [NBP2-61431]

Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5814) [NBP2-61431] - Staining of rat cerebral cortex shows strong positivity in neuronal cell bodies and in neuropil.
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Applications for beta-III Tubulin Antibody (CL5814)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:50000 - 1:100000

Immunohistochemistry-Paraffin

1:50000 - 1:100000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: PFA/Triton X-100

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: beta-III Tubulin

Beta-III Tubulin is abundant in the central and peripheral nervous systems where it is prominently expressed during fetal and postnatal development. Beta III Tubulin is often referred to as TUJ1 because Tuj-1 is the clone name of the original monoclonal antibody. The Tuj1 clone is such a popular neuronal marker that Tuj-1 is now used as a synonym for beta III tubulin. The distribution of Tubulin Beta 3 is neuron-associated, exhibiting distinct temporospatial gradients according to the regional neuroepithelia of origin. However, transient expression of the Tubulin Beta 3 protein is also present in the subventricular zones of the CNS comprising putative neuronal- and/or glial precursor cells, as well as in Kulchitsky neuroendocrine cells of the fetal respiratory epithelium. This temporally restricted, potentially non-neuronal expression may have implications in the identification of presumptive neurons derived from embryonic stem cells. Mutation on beta-tubulin has been linked to anti-cancer drug resistance in lung cancers.

Alternate Names

beta-Tubulin III, Beta3-tubulin, betaIII Tubulin, Class III beta-tubulin, TUBB3, Tubulin Beta 3, Tubulin beta-4, Tubulin beta-III

Gene Symbol

TUBB3

Additional beta-III Tubulin Products

Product Documents for beta-III Tubulin Antibody (CL5814)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for beta-III Tubulin Antibody (CL5814)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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