beta Sarcoglycan Antibody (1C10)

Novus Biologicals, part of Bio-Techne | Catalog # H00006443-M02

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Product Datasheet / COA / SDS

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1C10

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

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Product Specifications

Immunogen

SGCB (NP_000223, 87 a.a. ~ 196 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. WAVIRIGPNGCDSMEFHESGLLRFKQVSDMGVIHPLYKSTVGGRRNENLVITGNNQPIVFQQGTTKLSVENNKTSITSDIGMQFFDPRTQNILFSTDYETHEFHLPSGVK

Specificity

SGCB (1C10)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for beta Sarcoglycan Antibody (1C10)

Western Blot: beta Sarcoglycan Antibody (1C10) [H00006443-M02]

Western Blot: beta Sarcoglycan Antibody (1C10) [H00006443-M02] - SGCB monoclonal antibody (M02), clone 1C10 Analysis of SGCB expression in MCF-7.

Immunocytochemistry/ Immunofluorescence: beta Sarcoglycan Antibody (1C10) [H00006443-M02]

Immunocytochemistry/Immunofluorescence: beta Sarcoglycan Antibody (1C10) [H00006443-M02] - Analysis of monoclonal antibody to SGCB on MCF-7 cell . Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: beta Sarcoglycan Antibody (1C10) [H00006443-M02]

Immunohistochemistry-Paraffin: beta Sarcoglycan Antibody (1C10) [H00006443-M02] - Analysis of monoclonal antibody to SGCB on formalin-fixed paraffin-embedded human heart. Antibody concentration 3 ug/ml.

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Applications for beta Sarcoglycan Antibody (1C10)

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: beta-Sarcoglycan

The dystrophin-glycoprotein complex (DGC) is a multisubunit protein complex that spans the sarcolemma and provides structural linkage between the subsarcolemmal cytoskeleton and the extracellular matrix of muscle cells. There are 3 main subcomplexes of the DGC: the cytoplasmic proteins dystrophin (DMD; MIM 300377) and syntrophin (SNTA1; MIM 601017), the alpha- and beta-dystroglycans (see MIM 128239), and the sarcoglycans (see, e.g., SGCA; MIM 600119) (Crosbie et al., 2000 [PubMed 10942431]).[supplied by OMIM]

Alternate Names

43DAG, A3b, beta Sarcoglycan, Beta-SG, Sarcoglycan beta, SGC, SGCB

Entrez Gene IDs

6443 (Human)

Gene Symbol

SGCB

OMIM

600900 (Human)

UniProt

NP_000223

Additional beta-Sarcoglycan Products

Product Documents for beta Sarcoglycan Antibody (1C10)

Product Datasheets

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COA

SDS

Product Specific Notices for beta Sarcoglycan Antibody (1C10)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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