Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3D5

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all beta-TrCP1/BTRC Antibodies »

Product Specifications

Immunogen

Human fragment corresponding to amino acids 52-354 of human BTRC (NP_378663) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

68.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for beta-TrCP1/BTRC Antibody (OTI3D5) - Azide and BSA Free

Western Blot: beta-TrCP1/BTRC Antibody (OTI3D5)Azide and BSA Free [NBP2-71401]

Western Blot: beta-TrCP1/BTRC Antibody (OTI3D5)Azide and BSA Free [NBP2-71401]

Western Blot: beta-TrCP1/BTRC Antibody (OTI3D5) - Azide and BSA Free [NBP2-71401] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY betaTrCP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-betaTrCP.
Immunocytochemistry/ Immunofluorescence: beta-TrCP1/BTRC Antibody (OTI3D5) - Azide and BSA Free [NBP2-71401]

Immunocytochemistry/ Immunofluorescence: beta-TrCP1/BTRC Antibody (OTI3D5) - Azide and BSA Free [NBP2-71401]

Immunocytochemistry/Immunofluorescence: beta-TrCP1/BTRC Antibody (OTI3D5) - Azide and BSA Free [NBP2-71401] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY betaTrCP.

Applications for beta-TrCP1/BTRC Antibody (OTI3D5) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: beta-TrCP1/BTRC

betaTrCP encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class; in addition to an F-box, this protein contains multiple WD-40 repeats. This protein is homologous to Xenopus bTrCP1, yeast Met30, Neurospora Scon2 and Drosophila Slimb proteins. It interacts with HIV-1 Vpu and connects CD4 to the proteolytic machinery. It also associates specifically with phosphorylated IkappaBalpha and beta-catenin destruction motifs, probably functioning in multiple transcriptional programs by activating the NF-kappaB pathway and inhibiting the beta-catenin pathway. [provided by RefSeq]

Long Name

Beta-Transducin Repeat Containing E3 Ubiquitin Protein Ligase

Alternate Names

betaTrCP1, BTRC, BTRCP, E3RSIkappaB, FBW1A, FBXW1A, FWD1

Gene Symbol

BTRC

Additional beta-TrCP1/BTRC Products

Product Documents for beta-TrCP1/BTRC Antibody (OTI3D5) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for beta-TrCP1/BTRC Antibody (OTI3D5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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