beta-TrCP1/BTRC Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-33301

Novus Biologicals, part of Bio-Techne
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NBP1-33301
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Predicted:

Bovine (99%), Mouse (99%), Xenopus (96%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all beta-TrCP1/BTRC Antibodies »

Product Summary for beta-TrCP1/BTRC Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human beta-TrCP1/BTRC. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (96%).

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

69 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for beta-TrCP1/BTRC Antibody

Western Blot: beta-TrCP1/BTRC Antibody [NBP1-33301]

Western Blot: beta-TrCP1/BTRC Antibody [NBP1-33301]

Western Blot: beta-TrCP1/BTRC Antibody [NBP1-33301] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with beta-TrCP1 antibody diluted at 1:500.
Immunohistochemistry-Paraffin: beta-TrCP1/BTRC Antibody [NBP1-33301]

Immunohistochemistry-Paraffin: beta-TrCP1/BTRC Antibody [NBP1-33301]

Immunohistochemistry-Paraffin: beta-TrCP1/BTRC Antibody [NBP1-33301] - Paraffin-embedded rat fore brain. beta-TrCP antibody [N1C1] dilution: 1:250.
beta-TrCP1/BTRC Antibody

Western Blot: beta-TrCP1/BTRC Antibody [NBP1-33301] -

Western Blot: beta-TrCP1/BTRC Antibody [NBP1-33301] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with beta-TrCP1/BTRC antibody [N1C1] (NBP1-33301) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for beta-TrCP1/BTRC Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: beta-TrCP1/BTRC

betaTrCP encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class; in addition to an F-box, this protein contains multiple WD-40 repeats. This protein is homologous to Xenopus bTrCP1, yeast Met30, Neurospora Scon2 and Drosophila Slimb proteins. It interacts with HIV-1 Vpu and connects CD4 to the proteolytic machinery. It also associates specifically with phosphorylated IkappaBalpha and beta-catenin destruction motifs, probably functioning in multiple transcriptional programs by activating the NF-kappaB pathway and inhibiting the beta-catenin pathway. [provided by RefSeq]

Long Name

Beta-Transducin Repeat Containing E3 Ubiquitin Protein Ligase

Alternate Names

betaTrCP1, BTRC, BTRCP, E3RSIkappaB, FBW1A, FBXW1A, FWD1

Gene Symbol

BTRC

Additional beta-TrCP1/BTRC Products

Product Documents for beta-TrCP1/BTRC Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for beta-TrCP1/BTRC Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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