beta Tubulin Antibody (1-B11-R)

Western Blot: beta Tubulin Antibody (1-B11-R) [NBP3-32080]

Western Blot: beta Tubulin Antibody (1-B11-R) [NBP3-32080] - Western blot analysis of beta Tubulin on different lysates with Mouse anti-beta Tubulin antibody (NBP3-32080) at 1/5,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: MCF7 cell lysate
Lane 3: NCCIT cell lysate
Lane 4: PC-12 cell lysate
Lane 5: F9 cell lysate
Lane 6: NIH/3T3 cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 50 kDa
Observed band size: 50 kDa

Exposure time: 2 minutes;

10% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32080) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: beta Tubulin Antibody (1-B11-R) [NBP3-32080]

Immunohistochemistry: beta Tubulin Antibody (1-B11-R) [NBP3-32080] - Immunohistochemical analysis of paraffin-embedded human spleen tissue with Mouse anti-beta Tubulin antibody (NBP3-32080) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32080) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody (1-B11-R) [NBP3-32080]

Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody (1-B11-R) [NBP3-32080] - Immunocytochemistry analysis of HeLa cells labeling beta Tubulin with Mouse anti-beta Tubulin antibody (NBP3-32080) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Mouse anti-beta Tubulin antibody (NBP3-32080) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.