beta Tubulin Antibody (S11B) - Chimeric - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81064

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Lambda Clone # S11B

Format

Azide and BSA Free

Concentration

1 mg/ml

View all beta Tubulin Antibodies »

Product Specifications

Immunogen

Human MBP (microtubule-binding protein).

Specificity

Binds specifically to human beta Tubulin (no crossreactivity with alpha-tubulin).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Lambda

Scientific Data Images for beta Tubulin Antibody (S11B) - Chimeric - Azide and BSA Free

Western Blot: beta Tubulin Antibody (S11B)ChimericAzide and BSA Free [NBP2-81064]

Western Blot: beta Tubulin Antibody (S11B)ChimericAzide and BSA Free [NBP2-81064]

Western Blot: beta Tubulin Antibody (S11B) [NBP2-81064] - Western Blot using anti-Beta-Tubulin antibody (S11B) [NBP2-81064]. HeLa (A), A431 (B), HEK293 (C) and MCF-7 (D) cell lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of S11B [NBP2-81064] at 0.01 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for Beta-Tubulin is ~54kDa. NBP2-81064 successfully detected human Beta-Tubulin in HeLa, A431, HEK293 and MCF-7 cell lysate samples.
Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody (S11B) - Chimeric - Azide and BSA Free [NBP2-81064]

Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody (S11B) - Chimeric - Azide and BSA Free [NBP2-81064]

Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody (S11B) [NBP2-81064] - Immunofluoresence staining of fixed HeLa cells with anti-Beta-tubulin antibody (S11B) [NBP2-81064]. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of S11B [NBP2-81064] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81064, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.
Immunohistochemistry-Paraffin: beta Tubulin Antibody (S11B) - Chimeric - Azide and BSA Free [NBP2-81064]

Immunohistochemistry-Paraffin: beta Tubulin Antibody (S11B) - Chimeric - Azide and BSA Free [NBP2-81064]

Immunohistochemistry-Paraffin: beta Tubulin Antibody (S11B) [NBP2-81064] - Immunohistochemical staining of human cerebral cortex tissue using [NBP2-81064]. Anti-Beta Tubulin staining of paraffin embedded human cerebral cortex tissue using the rabbit-chimeric version of S11B [NBP2-81064]. Antigen retreival was acheived by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP2-81064 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Staining of neuronal cell bodies and their processes may be observed. Recommended concentration, 2-4 ug/ml.
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Applications for beta Tubulin Antibody (S11B) - Chimeric - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This full-length, chimeric rabbit antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques. This antibody binds to beta-tubulin. Tubulin is the major constituent of microtubules. The beta-chain has an exchangeable GTP-binding site.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: beta Tubulin

Beta Tubulin antibodies are useful as loading controls for Western Blotting and ELISA, as well as in studies invlolving cell structure and division. Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and in the cytoskeleton.

Long Name

Tubulin beta chain

Alternate Names

OK/SW-cl.56, TUBB, TUBB5

Gene Symbol

TUBB

Additional beta Tubulin Products

Product Documents for beta Tubulin Antibody (S11B) - Chimeric - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for beta Tubulin Antibody (S11B) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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