BHMT Antibody (OTI3E11)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-02349

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI3E11

Concentration

0.93 mg/ml

View all BHMT Antibodies »

Product Summary for BHMT Antibody (OTI3E11)

Immunogen

Full length human recombinant protein of human BHMT (NP_001704) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BHMT Antibody (OTI3E11)

Western Blot: BHMT Antibody (OTI3E11) [NBP2-02349]

Western Blot: BHMT Antibody (OTI3E11) [NBP2-02349]

Western Blot: BHMT Antibody (3E11) [NBP2-02349] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BHMT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BHMT.
Immunocytochemistry/Immunofluorescence: BHMT Antibody (3E11) [NBP2-02349] Staining of COS7 cells transiently transfected by pCMV6-ENTRY BHMT.
Immunohistochemistry-Paraffin: BHMT Antibody (OTI3E11) [NBP2-02349]

Immunohistochemistry-Paraffin: BHMT Antibody (OTI3E11) [NBP2-02349]

Immunohistochemistry-Paraffin: BHMT Antibody (3E11) [NBP2-02349] - Staining of paraffin-embedded liver tissue using anti-BHMT mouse monoclonal antibody.
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Applications for BHMT Antibody (OTI3E11)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:50

Western Blot

1:200
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.93 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: BHMT

This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed.

Alternate Names

betaine homocysteine methyltransferase, betaine-homocysteine methyltransferase, betaine--homocysteine S-methyltransferase, betaine--homocysteine S-methyltransferase 1, BHMT1, EC 2.1.1, EC 2.1.1.5

Entrez Gene IDs

635 (Human)

Gene Symbol

BHMT

Additional BHMT Products

Product Documents for BHMT Antibody (OTI3E11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for BHMT Antibody (OTI3E11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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