BHMT Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-00161
![Immunohistochemistry-Paraffin: BHMT Antibody [NBP1-00161]](https://resources.bio-techne.com/images/products/BHMT-Antibody-Immunohistochemistry-Paraffin-NBP1-00161-img0004.jpg "Immunohistochemistry-Paraffin: BHMT Antibody [NBP1-00161]")
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse
Predicted:
Canine (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-EQQLKELFEKQK corresponding to C-Terminus according to NP_001704.1 .
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for BHMT Antibody
![Western Blot: BHMT Antibody [NBP1-00161]](https://resources.bio-techne.com/images/products/BHMT-Antibody-Western-Blot-NBP1-00161-img0003.jpg "Western Blot: BHMT Antibody [NBP1-00161]")
Western Blot: BHMT Antibody [NBP1-00161]
Western Blot: BHMT Antibody [NBP1-00161] - Rat Liver lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.![Immunohistochemistry-Paraffin: BHMT Antibody [NBP1-00161]](https://resources.bio-techne.com/images/products/BHMT-Antibody-Immunohistochemistry-Paraffin-NBP1-00161-img0005.jpg "Immunohistochemistry-Paraffin: BHMT Antibody [NBP1-00161]")
Immunohistochemistry-Paraffin: BHMT Antibody [NBP1-00161]
Immunohistochemistry-Paraffin: BHMT Antibody [NBP1-00161] - Human Kidney tissue. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.![Western Blot: BHMT Antibody [NBP1-00161]](https://resources.bio-techne.com/images/products/BHMT-Antibody-Western-Blot-NBP1-00161-img0001.jpg "Western Blot: BHMT Antibody [NBP1-00161]")
Western Blot: BHMT Antibody [NBP1-00161]
Western Blot: BHMT Antibody [NBP1-00161] - HEK293 overexpressing BHMT and probed with this antibody (mock transfection in first lane).Applications for BHMT Antibody
Application
Recommended Usage
Immunohistochemistry
5 ug/mL
Immunohistochemistry-Paraffin
5 ug/mL
Peptide ELISA
Detection limit 1:16000
Western Blot
0.03 - 0.1 ug/mL
Application Notes
Approx 45 kDa band observed in Rat Liver lysates (calculated MW of 44.9 kDa according to human NP_001704.1, 45.0 kDa according to mouse NP_057877.1 and rat NP_110477.1). This product has been successfully used in WB on Mouse (PMID:28605831, 23807810 and 22209966). In transfected HEK293 transiently expressing BHMT a band of approx. 49kDa is observed. This band is not observed in the non-transfected HEK293.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: BHMT
Alternate Names
betaine homocysteine methyltransferase, betaine-homocysteine methyltransferase, betaine--homocysteine S-methyltransferase, betaine--homocysteine S-methyltransferase 1, BHMT1, EC 2.1.1, EC 2.1.1.5
Gene Symbol
BHMT
UniProt
Additional BHMT Products
Product Documents for BHMT Antibody
Product Specific Notices for BHMT Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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