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BHMT2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85826

Novus Biologicals, part of Bio-Techne
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NBP1-85826
NBP1-85826-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: FTFSASEDNMESKWEDVNAAACDLAREVAGKGDALVAGGICQTSIYKYQKDEA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BHMT2 Antibody

Western Blot: BHMT2 Antibody [NBP1-85826]

Western Blot: BHMT2 Antibody [NBP1-85826]

Western Blot: BHMT2 Antibody [NBP1-85826] - Lane 1: Mouse liver tissue lysate Lane 2: Rat liver tissue lysate
Immunohistochemistry-Paraffin: BHMT2 Antibody [NBP1-85826]

Immunohistochemistry-Paraffin: BHMT2 Antibody [NBP1-85826]

Immunohistochemistry-Paraffin: BHMT2 Antibody [NBP1-85826] - Staining in human kidney and lymph node tissues using anti-BHMT2 antibody. Corresponding BHMT2 RNA-seq data are presented for the same tissues.
Western Blot: BHMT2 Antibody [NBP1-85826]

Western Blot: BHMT2 Antibody [NBP1-85826]

Western Blot: BHMT2 Antibody [NBP1-85826] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue

Applications for BHMT2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BHMT2

Homocysteine is a sulfur-containing amino acid that plays a crucial role in methylation reactions. Transfer of the methyl group from betaine to homocysteine creates methionine, which donates the methyl group to methylate DNA, proteins, lipids, and other intracellular metabolites. The protein encoded by this gene is one of two methyl transferases that can catalyze the transfer of the methyl group from betaine to homocysteine. Anomalies in homocysteine metabolism have been implicated in disorders ranging from vascular disease to neural tube birth defects such as spina bifida. [provided by RefSeq]

Alternate Names

betaine-homocysteine methyltransferase 2, betaine--homocysteine S-methyltransferase 2, EC 2.1.1.5, FLJ20001

Gene Symbol

BHMT2

Additional BHMT2 Products

Product Documents for BHMT2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for BHMT2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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