Biglycan Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-55407
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Bovine
Cited:
Human, Rat, Bovine
Predicted:
Canine (100%). Backed by our 100% Guarantee.
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Peptide ELISA
Cited:
ELISA
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Biglycan Antibody is made to a peptide with sequence C-DRLAIQFGNYKK corresponding to C-Terminus according to NP_001702.1.
Reactivity Notes
Bovine reactivity reported in scientific literature (PMID: 22427827).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Theoretical MW
41.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Biglycan Antibody
![Immunocytochemistry/ Immunofluorescence: Biglycan Antibody [NB100-55407]](https://resources.bio-techne.com/images/products/Biglycan-Antibody-Immunocytochemistry-Immunofluorescence-NB100-55407-img0002.jpg "Immunocytochemistry/ Immunofluorescence: Biglycan Antibody [NB100-55407]")
Immunocytochemistry/ Immunofluorescence: Biglycan Antibody [NB100-55407]
Immunocytochemistry/Immunofluorescence: Biglycan Antibody [NB100-55407] - Analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing Golgi/ER staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).![Immunocytochemistry/ Immunofluorescence: Biglycan Antibody [NB100-55407]](https://resources.bio-techne.com/images/products/Biglycan-Antibody-Immunocytochemistry-Immunofluorescence-NB100-55407-img0001.jpg "Immunocytochemistry/ Immunofluorescence: Biglycan Antibody [NB100-55407]")
Immunocytochemistry/ Immunofluorescence: Biglycan Antibody [NB100-55407]
Immunocytochemistry/Immunofluorescence: Biglycan Antibody [NB100-55407] - Analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing Golgi staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).Applications for Biglycan Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
10 ug/mL
Immunohistochemistry
1:10 - 1:500
Peptide ELISA
Detection limit 1:2000
Application Notes
IHC successful usage on human achilles tendons is reported in scientific literature (PMID: 23001016). Use in ELISA reported in scientific literature (PMID:26694746)
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Biglycan
Alternate Names
BGN, DSPG1, PG-S1, SLRR1A
Gene Symbol
BGN
UniProt
Additional Biglycan Products
Product Documents for Biglycan Antibody
Product Specific Notices for Biglycan Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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