BIK Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56109

Novus Biologicals, part of Bio-Techne
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NB100-56109
NB100-56109SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all BIK Antibodies »

Product Specifications

Immunogen

Recombinant full-length human Bik protein was used as immunogen.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BIK Antibody

Immunohistochemistry-Paraffin: BIK Antibody [NB100-56109]

Immunohistochemistry-Paraffin: BIK Antibody [NB100-56109]

Immunohistochemistry-Paraffin: BIK Antibody [NB100-56109] - Tissue section of human Non-Hodgkin's lymphoma (NHL) stained for Bik expression using this antibody at 1:2000. Hematoxylin-eosin counterstain.
Immunohistochemistry-Paraffin: BIK Antibody [NB100-56109]

Immunohistochemistry-Paraffin: BIK Antibody [NB100-56109]

Immunohistochemistry-Paraffin: BIK Antibody [NB100-56109] - Tissue section of human kidney stained for Bik expression using this antibody at 1:2000. A high level of Bik signal was seen in the distal collecting tubules, the glomeruli are primarily negative. Hematoxylin-eosin counterstain.

Applications for BIK Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:1000-1:5000

Immunoprecipitation

1:50-1:200

Western Blot

1:1000-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BIK

The Bcl-2 family of apoptosis-related genes plays central roles in regulating apoptotic pathways (reviewed in Thomadaki and Scorilas, 2006). Regulation of cell death through apoptosis is critical for the maintenance of homeostasis, defense against infectious agents, and normal development. Bcl-2 family proteins regulate apoptosis primarily through the regulation of mitochondrial outer membrane permeability. In mammals, the family consists of both antiapoptotic (prosurvival) and proapoptotic (prodeath) members. Cellular homeostasis is thought to be dependent on a balance between the actions of antiapoptotic and proapoptotic proteins. Bcl-2 family proteins can be divided into 3 main subfamilies on the basis of their function and the content of their Bcl-2 homology (BH) domains, for example: 1) Antiapoptotic: Bcl-2, Bcl-xL, Bcl-W, A1, and Mcl-1 2) Proapoptotic (multidomain): Bax, Bak, and Bok. 3) BH3-only (proapoptotic): Bad, Bcl-xS, Bid, Bik, Bim, Blk, Bmf, Bnip, Noxa, and Puma. Antiapoptotic members inhibit cells from undergoing apoptosis, whereas proapoptotic and BH3-only subfamily members promote apoptosis. There are 4 BH domains (1-4) conserved among Bcl-2 family proteins. The BH domains are important for function as well as for heterodimerization between family members. Typical antiapoptotic family members have all four BH domains (1-4), whereas proapoptotic (multidomain) members have BH1, 2 and 3 domains and BH3-only members have only the BH3 domain. Overall, the relative ratio of antiapoptotic and proapoptotic proteins determines the susceptibility of a cell to various apoptotic stimuli. Many Bcl-2 family proteins are differentially expressed in various malignancies and some are useful prognostic biomarkers. Antiapoptotic proteins are often elevated in diverse cancers and have the potential to confer resistance to both endogenous cell death stimuli and cancer treatments. Alterations in the ratio or levels of Bcl-2 family proteins have been also associated with nonmalignant diseases including neurodegenerative diseases, autoimmune diseases, AIDs, Down syndrome, cardiovascular diseases, diabetes, glomerulonephritis, and muscular dystrophy. This antibody recognizes Bik. Human Bik is a 160 amino acid protein, GenBank no. NP_001188.1.

Long Name

Bcl-2 Interacting Killer

Alternate Names

BIP1, BP4, NBK

Entrez Gene IDs

638 (Human); 12124 (Mouse); 114496 (Rat)

Gene Symbol

BIK

Additional BIK Products

Product Documents for BIK Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for BIK Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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