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Biliverdin Reductase B/BLVRB Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83435

Novus Biologicals, part of Bio-Techne
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NBP1-83435
NBP1-83435-25ul

Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (93%), Rat (92%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for Biliverdin Reductase B/BLVRB Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: HIRMHKVLRESGLKYVAVMPPHIGDQPLTGAYTVTLDGRGPSRVISKHDLGHFMLRCLTTDEYDGHSTYPSHQYQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Biliverdin Reductase B/BLVRB Antibody

Immunohistochemistry-Paraffin: Biliverdin Reductase B/BLVRB Antibody [NBP1-83435]

Immunohistochemistry-Paraffin: Biliverdin Reductase B/BLVRB Antibody [NBP1-83435]

Immunohistochemistry-Paraffin: Biliverdin Reductase B/BLVRB Antibody [NBP1-83435] - Staining of human colon, liver, small intestine and spleen using Anti-BLVRB antibody NBP1-83435 (A) shows similar protein distribution across tissues to independent antibody NBP1-83434 (B).
Western Blot: Biliverdin Reductase B/BLVRB Antibody [NBP1-83435]

Western Blot: Biliverdin Reductase B/BLVRB Antibody [NBP1-83435]

Western Blot: Biliverdin Reductase B/BLVRB Antibody [NBP1-83435] - Analysis in human cell lines A-549 and U-251MG. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: Biliverdin Reductase B/BLVRB Antibody [NBP1-83435]

Immunocytochemistry/ Immunofluorescence: Biliverdin Reductase B/BLVRB Antibody [NBP1-83435]

Immunocytochemistry/Immunofluorescence: Biliverdin Reductase B/BLVRB Antibody [NBP1-83435] - Staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.

Applications for Biliverdin Reductase B/BLVRB Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Biliverdin Reductase B/BLVRB

The final step in heme metabolism in mammals is catalyzed by the cytosolic biliverdin reductase enzymes A and B (EC1.3.1.24).(supplied by OMIM)

Alternate Names

BLVRB, BVRB, FLR, GHBP

Gene Symbol

BLVRB

Additional Biliverdin Reductase B/BLVRB Products

Product Documents for Biliverdin Reductase B/BLVRB Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Biliverdin Reductase B/BLVRB Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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