Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1C2H4

Format

BSA Free

Concentration

1 mg/ml

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Product Specifications

Immunogen

Antibody was raised against a recombinant protein corresponding to amino acids 2 to 198 of human Bim.

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Bim Antibody (1C2H4) - BSA Free

Western Blot: Bim Antibody (1C2H4)BSA Free [NBP1-75511]

Western Blot: Bim Antibody (1C2H4)BSA Free [NBP1-75511]

Western Blot: Bim Antibody (1C2H4) [NBP1-75511] - Western blot analysis of 5 ng of Bim recombinant protein with Bim antibody at 1 ug/ml.
Bim Antibody (1C2H4) - BSA Free

Immunocytochemistry/ Immunofluorescence: Bim Antibody (1C2H4) - BSA Free [NBP1-75511] -

Immunocytochemistry/ Immunofluorescence: Bim Antibody (1C2H4) - BSA Free [NBP1-75511] - Immunofluorescence of Bim in K562 cells with Bim antibody at 20 u/mL.
Bim Antibody (1C2H4) - BSA Free

Immunocytochemistry/ Immunofluorescence: Bim Antibody (1C2H4) - BSA Free [NBP1-75511] -

Immunocytochemistry/ Immunofluorescence: Bim Antibody (1C2H4) - BSA Free [NBP1-75511] - Immunocytochemistry of Bim in K562 cells with Bim antibody at 10 u/mL.

Applications for Bim Antibody (1C2H4) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

10-20 ug/ml

Western Blot

1 ug/ml

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 degrees C. Avoid freeze/thaw cycles.

Background: Bim

Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. Bim is another BH3 domain containing protein which can induce apoptosis. Bim interacts with diverse members in the pro-survival Bcl-2 sub-family including Bcl-2, Bcl-xL and Bcl-w. The messenger RNA of Bim is ubiquitously expressed in multiple tissues and cell lines.

Alternate Names

BAM, bcl-2 interacting mediator of cell death, bcl-2 interacting protein Bim, Bcl2-interacting mediator of cell death, Bcl2-L-11, BCL2-like 11 (apoptosis facilitator), bcl-2-like protein 11, bcl-2-related ovarian death agonist, BIM-alpha6, BIM-beta6, BIM-beta7, BIMBOD, BimEL, BimL

Entrez Gene IDs

10018 (Human); 12125 (Mouse); 64547 (Rat)

Gene Symbol

BCL2L11

UniProt

O43521 (Human)

Additional Bim Products

Product Documents for Bim Antibody (1C2H4) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Bim Antibody (1C2H4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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