BIN1 Antibody (12A)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-21689
Key Product Details
Species Reactivity
Human
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 12A
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
BIN1 Antibody (12A) was produced in mouse by repeated immunizations with 12A exon BIN1 protein followed by hybridoma development. (Uniprot: O00499)
Reactivity Notes
Cross-reactivity with BIN1 from other sources has not been determined
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
This antibody was purified from clarified mouse ascetic fluid by Protein A chromatography followed by extensive dialysis against the buffer stated above
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for BIN1 Antibody (12A)
![Western Blot: BIN1 Antibody (12A) [NBP2-21689]](https://resources.bio-techne.com/images/products/BIN1-Antibody-12A-Western-Blot-NBP2-21689-img0001.jpg "Western Blot: BIN1 Antibody (12A) [NBP2-21689]")
Western Blot: BIN1 Antibody (12A) [NBP2-21689]
Western Blot: BIN1 Antibody (12A) [NBP2-21689] - Mouse Anti-BIN-1 Antibody. Lane 1: LNCap. Lane 2: WM1341D. Lane 3: WM164. Lane 4: WM983A. Lane 5: WM793. Lane 6: WM239A. Lane 7: WM35. Load: 35 ug per lane. BIN-1 antibody at 1:400 for overnight at 4C. Secondary antibody: Dye800 mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% Blocking Buffer overnight at 4C.
BIN1 Antibody (12A)
Western Blot of Rabbit Anti-BIN1 Antibody (12A). Lane 1: WM239A lysate. Lane 2: HEK293 lysateApplications for BIN1 Antibody (12A)
Application
Recommended Usage
ELISA
1:5000-1:50000
Western Blot
1:500-1:1500
Application Notes
This product has been tested for use in ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: BIN1
Alternate Names
AMPH2, Amphiphysin II, Amphiphysin-like protein, AMPHLDKFZp547F068, box dependant MYC interacting protein 1, bridging integrator 1Box-dependent myc-interacting protein 1, MGC10367, myc box-dependent-interacting protein 1, SH3P9
Gene Symbol
BIN1
UniProt
Additional BIN1 Products
Product Documents for BIN1 Antibody (12A)
Product Specific Notices for BIN1 Antibody (12A)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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