Biotin (Vitamin B7 or Vitamin H) Antibody (Hyb-8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-32811

Novus Biologicals, part of Bio-Techne
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NBP2-32811-0.1mg
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Key Product Details

Species Reactivity

Validated:

Non-species specific

Cited:

Bovine

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, In-situ Hybridization, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # Hyb-8

Concentration

0.2 mg/ml

View all Biotin (Vitamin B7 or Vitamin H) Antibodies »

Product Specifications

Immunogen

Biotinylated sheep immunoglobulin

Localization

Depends on the target

Specificity

It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This monoclonal antibody is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

244 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34646)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Biotin (Vitamin B7 or Vitamin H) Antibody (Hyb-8)

Immunohistochemistry-Paraffin: Biotin (Vitamin B7 or Vitamin H) Antibody (Hyb-8) [NBP2-32811]

Immunohistochemistry-Paraffin: Biotin (Vitamin B7 or Vitamin H) Antibody (Hyb-8) [NBP2-32811]

Immunohistochemistry-Paraffin: Biotin (Vitamin B7 or Vitamin H) Antibody (Hyb-8) [NBP2-32811] - Formalin-fixed, paraffin-embedded human tonsil stained with biotinylated Lambda Light Chain probe followed by anti-biotin MAb (Hyb-8). Note cell membrane & cytoplasmic staining.

Applications for Biotin (Vitamin B7 or Vitamin H) Antibody (Hyb-8)

Application
Recommended Usage

Flow Cytometry

0.5-1 ug/million cells

Immunocytochemistry/ Immunofluorescence

0.5-1 ug/ml

Immunohistochemistry-Paraffin

0.5-1 ug/ml

Western Blot

0.5-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Biotin (Vitamin B7 or Vitamin H)

Alternate Names

58-85-5, coenzyme R, d-biotin, Vitamin B7, vitamin H

Additional Biotin (Vitamin B7 or Vitamin H) Products

Product Documents for Biotin (Vitamin B7 or Vitamin H) Antibody (Hyb-8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Biotin (Vitamin B7 or Vitamin H) Antibody (Hyb-8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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