Biotin (Vitamin B7 or Vitamin H) Antibody (SPM375) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34771
![Immunohistochemistry-Paraffin: Biotin (Vitamin B7 or Vitamin H) Antibody (SPM375) - Azide and BSA Free [NBP2-34771]](https://resources.bio-techne.com/images/products/Biotin-Vitamin-B7-or-Vitamin-H-Antibody-SPM375-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34771-img0001.jpg "Immunohistochemistry-Paraffin: Biotin (Vitamin B7 or Vitamin H) Antibody (SPM375) - Azide and BSA Free [NBP2-34771]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Non-species specific
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SPM375
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Biotinylated sheep immunoglobulin
Localization
Depends on the target
Specificity
It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This monoclonal antibody is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
244 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-32812).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images
Immunohistochemistry-Paraffin: Biotin (Vitamin B7 or Vitamin H) Antibody (SPM375) - Azide and BSA Free [NBP2-34771]
Immunohistochemistry-Paraffin: Biotin (Vitamin B7 or Vitamin H) Antibody (SPM375) - Azide and BSA Free [NBP2-34771] - Formalin-fixed, paraffin-embedded human Tonsil stained with biotinylated Lambda Light Chain probe followed by anti-biotin Monoclonal Antibody (SPM375).Applications
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Biotin (Vitamin B7 or Vitamin H)
Alternate Names
58-85-5, coenzyme R, d-biotin, Vitamin B7, vitamin H
Additional Biotin (Vitamin B7 or Vitamin H) Products
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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