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Biotinidase/BTD Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-97093

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human Biotinidase/BTD (Uniprot#: P43251; Met1-Asp543).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Biotinidase/BTD Antibody

Western Blot: Biotinidase/BTD Antibody [NBP2-97093]

Western Blot: Biotinidase/BTD Antibody [NBP2-97093]

Western Blot: Biotinidase/BTD Antibody [NBP2-97093] - Anti-Biotinidase/BTD rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 61 kDa. Observed band size: 48 kDa
Immunohistochemistry-Paraffin: Biotinidase/BTD Antibody [NBP2-97093]

Immunohistochemistry-Paraffin: Biotinidase/BTD Antibody [NBP2-97093]

Immunohistochemistry-Paraffin: Biotinidase/BTD Antibody [NBP2-97093] - Immunochemical staining of human Biotinidase/BTD in human liver with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).
Immunohistochemistry-Paraffin: Biotinidase/BTD Antibody [NBP2-97093]

Immunohistochemistry-Paraffin: Biotinidase/BTD Antibody [NBP2-97093]

Immunohistochemistry-Paraffin: Biotinidase/BTD Antibody [NBP2-97093] - Immunochemical staining of human Biotinidase/BTD in human kidney with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).

Applications for Biotinidase/BTD Antibody

Application
Recommended Usage

ELISA

1:25000-1:50000

Immunohistochemistry

1:2500-1:10000

Immunohistochemistry-Paraffin

1:2500-1:10000

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Biotinidase/BTD

Biotinidase functions to recycle biotin in the body by cleaving biocytin (biotin-epsilon-lysine), a normal product of carboxylase degradation, resulting in regeneration of free biotin. Biotinidase has also been shown to have biotinyl-transferase activity. Defects in the biotinidase gene cause multiple carboxylase deficiency. [provided by RefSeq]

Alternate Names

Biotinase, BTD

Gene Symbol

BTD

Additional Biotinidase/BTD Products

Product Documents for Biotinidase/BTD Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Biotinidase/BTD Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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