BLIMP1/PRDM1 Antibody (3H2-E8) [DyLight 550]
Novus Biologicals, part of Bio-Techne | Catalog # NB600-235R
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Porcine
Applications
Chromatin Immunoprecipitation (ChIP), ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
DyLight 550 (Excitation = 562 nm, Emission = 576 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 3H2-E8
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A fragment of mouse Blimp-1 corresponding to residues 199-409. [UniProt# Q60636]
Reactivity Notes
Porcine usage reported in customer reviews
Localization
Nuclear
Marker
Plasma Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for BLIMP1/PRDM1 Antibody (3H2-E8) [DyLight 550]
Immunocytochemistry/Immunofluorescence: BLIMP1/PRDM1 Antibody (3H2-E8) [DyLight 550] [NB600-235R] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-BLIMP1/PRDM1 (3H2-E8) conjugated to DyLight 550 [NB600-235R] at 20ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Applications for BLIMP1/PRDM1 Antibody (3H2-E8) [DyLight 550]
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
Optimal dilutions of this antibody should be experimentally determined.
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Frozen
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: BLIMP1/PRDM1
Long Name
B Lymphocyte Induced Maturation Protein
Alternate Names
PRDM1, ZNFPR1A1
Gene Symbol
PRDM1
Additional BLIMP1/PRDM1 Products
Product Documents for BLIMP1/PRDM1 Antibody (3H2-E8) [DyLight 550]
Product Specific Notices for BLIMP1/PRDM1 Antibody (3H2-E8) [DyLight 550]
DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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